| Rank | Bib | Name | Affiliation | Time | Gap | Category |
| 1 | 384 | Grace Wilson | DDP Elite Cycling Team | 36:16. | 36:16 | W 3-4 |
| 2 | 382 | Emma Frost | Alto Velo Racing Club | 36:30. | 14 | W 3-4 |
| 3 | 372 | Yerany Morejon | SupraBars.com | 36:31. | 15 | W 3-4 |
| 4 | 383 | Julie Voss | University of Tennessee Cycling | 36:31. | s.t. | W 3-4 |
| 5 | 373 | Zoey Marks | DCC | 36:33. | 17 | W 3-4 |
| 6 | 379 | Sarah Godwin | Kingdom Elite Racing | 36:35. | 19 | W 3-4 |
| 7 | 375 | Melissa Gomez | Skyway P/b Domestique Coffee | 36:47. | 31 | W 3-4 |
| 8 | 376 | Emily Strange | Nashville Local | 39:30. | 3:14 | W 3-4 |
| 9 | 399 | Paige Montanye | 0 | 39:33. | 3:17 | W 3-4 |
| 10 | 377 | Yanilka Vega | Merlo & Fahrney Racing | 39:33. | s.t. | W 3-4 |
| 11 | 380 | Carla Bates | NGCA | 39:34. | s.t. | W 3-4 |
| 12 | 378 | Laina Hamilton | 0 | 39:34. | s.t. | W 3-4 |
| 13 | 370 | Tessa Leduc | Rocket Revolution | 39:34. | s.t. | W 4-5 |
| 14 | 381 | Iwona Martin | NGCA Elite p/b Tyler Perry Studios | 39:34. | s.t. | W 3-4 |
| DNS | 374 | Lauren McNatt | Piedmont University | | | W 3-4 |
