| Rank | Bib | Name | Affiliation | Time | Gap | Category |
| 1 | 25 | Kendall Ryan | L39ION OF LOS ANGELES | 1:00:04. | 1:00:04 | W P-1-2-3 |
| 2 | 30 | Harriet OWEN | UCI CTW: DNA PRO CYCLING | 1:00:04. | s.t. | W P-1-2-3 |
| 3 | 20 | Skylar Schneider | Miami Blazers | 1:00:04. | s.t. | W P-1-2-3 |
| 4 | 38 | Ivanie Blondin | Team Goldman Sachs ETFs Racing | 1:00:04. | s.t. | W P-1-2-3 |
| 5 | 11 | Claudia Marcks | Fearless Femme Racing pb Robertet | 1:00:05. | s.t. | W P-1-2-3 |
| 6 | 15 | KIARA LYLYK | UCI CTW: BONESHAKER PROJECT PRESENTED BY ROXO | 1:00:05. | s.t. | W P-1-2-3 |
| 7 | 61 | Melanie Wong | UTC - ButcherBox Cycling | 1:00:06. | s.t. | W P-1-2-3 |
| 8 | 13 | Coryn Labecki | UCI CTW: EF EDUCATION - CANNONDALE | 1:00:06. | s.t. | W P-1-2-3 |
| 9 | 36 | Paola Munoz | Team Goldman Sachs ETFs Racing | 1:00:06. | s.t. | W P-1-2-3 |
| 10 | 14 | Andrea Cyr | Texas Roadhouse Cycling | 1:00:07. | s.t. | W P-1-2-3 |
| 11 | 26 | Alexis Magner | L39ION OF LOS ANGELES | 1:00:07. | s.t. | W P-1-2-3 |
| 12 | 17 | Sammy Rose Dobrozsi | 0 | 1:00:07. | s.t. | W P-1-2-3 |
| 13 | 2 | Erica Carney | C4 Cycling | 1:00:07. | s.t. | W P-1-2-3 |
| 14 | 58 | Kaitlyn Agnew | UTC- ButcherBox Cycling | 1:00:07. | s.t. | W P-1-2-3 |
| 15 | 32 | Kaitlyn RAUWERDA | UCI CTW: DNA PRO CYCLING | 1:00:08. | s.t. | W P-1-2-3 |
| 16 | 37 | Taylor Kuyk-White | Team Goldman Sachs ETFs Racing | 1:00:08. | s.t. | W P-1-2-3 |
| 17 | 59 | Rachel Canning | UTC - ButcherBox Cycling | 1:00:08. | s.t. | W P-1-2-3 |
| 18 | 52 | Brittany Parffrey | Velocious Sport | 1:00:08. | s.t. | W P-1-2-3 |
| 19 | 42 | Aubrey Drummond | Team Goldman Sachs ETFs Racing | 1:00:08. | s.t. | W P-1-2-3 |
| 20 | 53 | Bethany Ingram | Bike Works p/b Fred Beans | 1:00:08. | s.t. | W P-1-2-3 |
| 21 | 44 | Shannon Koch | Kingdom Elite Racing | 1:00:08. | s.t. | W P-1-2-3 |
| 22 | 66 | Regina Doty | LA Sweat | 1:00:08. | s.t. | W P-1-2-3 |
| 23 | 19 | Samantha Schneider | Miami Blazers | 1:00:09. | s.t. | W P-1-2-3 |
| 24 | 33 | Heather FISCHER | UCI CTW: DNA PRO CYCLING | 1:00:09. | s.t. | W P-1-2-3 |
| 25 | 55 | Rebecca Lang | 0 | 1:00:09. | s.t. | W P-1-2-3 |
| 26 | 60 | Gillian Bennett | UTC- ButcherBox Cycling | 1:00:10. | s.t. | W P-1-2-3 |
| 27 | 31 | Holly BRECK | UCI CTW: DNA PRO CYCLING | 1:00:10. | s.t. | W P-1-2-3 |
| 28 | 51 | Debbie Milne | Supra Bars | 1:00:10. | s.t. | W P-1-2-3 |
| 29 | 23 | Elizabeth Harden | East Point Track Club | 1:00:11. | s.t. | W P-1-2-3 |
| 30 | 41 | Matilda Raynolds | | 1:00:11. | s.t. | W P-1-2-3 |
| 31 | 5 | Sara Rains | Velocious Sport | 1:00:11. | s.t. | W P-1-2-3 |
| 32 | 47 | Madeline Wayman | Kingdom Elite Racing | 1:00:12. | s.t. | W P-1-2-3 |
| 33 | 6 | Laura Van Gilder | Velocious Sport | 1:00:12. | s.t. | W P-1-2-3 |
| 34 | 45 | Valentina Hernandez | Kingdom Elite Racing | 1:00:12. | s.t. | W P-1-2-3 |
| 35 | 46 | Jeydy Praderas | Kingdom Elite Racing | 1:00:12. | s.t. | W P-1-2-3 |
| 36 | 7 | Melissa Gomez | 0 | 1:00:12. | s.t. | W P-1-2-3 |
| 37 | 21 | Julyn Aguila | Miami Blazers | 1:00:12. | s.t. | W P-1-2-3 |
| 38 | 57 | Stephanie Clark | North Georgia Cycling Association | 1:00:12. | s.t. | W P-1-2-3 |
| 39 | 62 | Julianna Rutecki | 0 | 1:00:12. | s.t. | W P-1-2-3 |
| 40 | 24 | Yarely Salazar | L39ION OF LOS ANGELES | 1:00:13. | s.t. | W P-1-2-3 |
| 41 | 3 | Amanda Alston | Newtype Cycling | 1:00:13. | s.t. | W P-1-2-3 |
| 42 | 40 | Ruth Allen | 0 | 1:00:14. | s.t. | W P-1-2-3 |
| 43 | 39 | Justine Thomas | Team Goldman Sachs ETFs Racing | 1:00:15. | 11 | W P-1-2-3 |
| 44 | 56 | Paige Kostanecki | Austin Outlaws | 1:00:18. | 14 | W P-1-2-3 |
| 45 | 27 | Llori Sharpe | L39ION OF LOS ANGELES | 1:00:21. | 17 | W P-1-2-3 |
| 46 | 29 | Kimberly LUCIE | UCI CTW: DNA PRO CYCLING | 1:00:27. | 23 | W P-1-2-3 |
| DNF | 4 | Adonays Vera | City Bikes | | | W P-1-2-3 |
| DNF | 10 | Ella Sibley | Fearless Femme Racing pb Robertet | | | W P-1-2-3 |
| DNF | 12 | Sophie Sutton | Fearless Femme Racing pb Robertet | | | W P-1-2-3 |
| DNF | 16 | MARJORIE BEMIS | BONESHAKER P/B ORANGE SEAL | | | W P-1-2-3 |
| DNF | 18 | Alexi Ramirez | Miami Blazers | | | W P-1-2-3 |
| DNF | 22 | Carlie Cooper | Work Hard Be Humble Cycling | | | W P-1-2-3 |
| DNF | 28 | Rachel LANGDON | UCI CTW: DNA PRO CYCLING | | | W P-1-2-3 |
| DNF | 48 | Luisa Parra | Kingdom Elite Racing | | | W P-1-2-3 |
| DNF | 49 | Mary joyce Monton | Austin Outlaws | | | W P-1-2-3 |
| DNF | 54 | Paige Montanye | N/A | | | W P-1-2-3 |
| DNF | 63 | Gabriella Arnold | LA Sweat | | | W P-1-2-3 |
| DNF | 64 | Ashley Weaver | | | | W P-1-2-3 |
| DNF | 65 | Mackenzie Myatt | Team Goldman Sachs ETFs Racing | | | W P-1-2-3 |
| DNS | 8 | Laura Jorgenson | Velocious Sport | | | W P-1-2-3 |
| DNS | 9 | Lauren McNatt | 0 | | | W P-1-2-3 |
| DNS | 34 | Claire Windsor | UCI CTW: CYNISCA CYCLING | | | W P-1-2-3 |
| DNS | 35 | Stephanie Halamek | Team Goldman Sachs ETFs Racing | | | W P-1-2-3 |
| DNS | 43 | Sarah Godwin | NGCA Elite p/b Tyler Perry Studios | | | W P-1-2-3 |
| DNS | 50 | Julie Voss | Flow Formulas | | | W P-1-2-3 |
