| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 246 | Makayla MACPHERSON | LUX/Sideshow p/b Specialized | 2:08:15. | 2:08:15 |
| 2 | 247 | Kaia SCHMID | LUX/sideshow p/b Specialized | 2:08:15. | s.t. |
| 3 | 245 | Olivia CUMMINS | LUX/sideshow p/b Specialized | 2:08:16. | 1 |
| 4 | 244 | Cassidy HICKEY | TeamTwenty24 | 2:08:17. | s.t. |
| 5 | 250 | Nekane POWELL | Liza Coaching | 2:08:17. | s.t. |
| 6 | 241 | Ajay BAKER | nine13sports p/b SingletonCoach | 2:16:49. | 8:34 |
| 7 | 252 | Mahika PETERSON | BYRDS | 2:16:50. | s.t. |
| 8 | 248 | Katherine SARKISOV | dcdevo Racing Academy | 2:16:50. | s.t. |
| 9 | 243 | Jordyn OLDENBURG | Liza Coaching | 2:16:50. | s.t. |
| 10 | 257 | Sarah GIBERTONI | Killington Mountain School / Bi | 2:16:51. | s.t. |
| 11 | 259 | Kayla YUENGLING | | 2:16:52. | s.t. |
| 12 | 255 | Olivia SANDOVAL | Kelly Benefit Strategies | 2:16:54. | 8:39 |
| @1Lap | 253 | Caleigh RASSER | Kelly Benefit Strategies/LSV | | |
| @2Lap | 258 | Lizzy GUNSALUS | CCB BikeReg | | |
| @2Lap | 251 | Ashley DAVIS | Velobrew | | |
| @2Lap | 242 | Emma BALDING | nine13sports p/b SingletonCoach | | |
| @2Lap | 249 | Sarah BANKS | nine13sports p/b SingletonCoach | | |
| @2Lap | 256 | Mallory BRYAN | TeamTwenty24 | | |
| DNF | 254 | Marissa CARTER | Team MACK Racing Elite | | |
