| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 12 | April BEARD | Trek Midwest Team | 41:04. | 10:33. | 4 | W 1-2 |
| 2 | 1 | Sydney GUAGLIARDO | | 41:33. | 10:49. | 4 | W 1-2 |
| 3 | 11 | Lauren HARKNESS | | 42:05. | 10:43. | 4 | W 1-2 |
| 4 | 5 | Maria LARKIN | The Call Up pb Bike Law | 42:05. | 10:20. | 4 | W 1-2 |
| 5 | 10 | Erin FELDHAUSEN | Trek Cyclocross Collective | 43:08. | 11:06. | 4 | W 1-2 |
| 6 | 14 | Kae TAKESHITA | Team Got Wind? | 45:35. | 11:49. | 4 | W 1-2 |
| 7 | 74 | Emma OSBORNE | Team Extreme | 45:48. | 11:29. | 4 | W 3-4 |
| 8 | 17 | Mary RANDALL | Chicago Cuttin Crew | 46:26. | 12:01. | 4 | W 1-2 |
| 9 | 8 | Jodie PRESTINE | Velo Femmes Racing | 46:41. | 11:45. | 4 | W 1-2 |
| 10 | 6 | Amanda SCHAAP | | 47:10. | 12:18. | 4 | W 1-2 |
| 11 | 4 | Courtney TANNER | The Pony Shop | 47:12. | 11:39. | 4 | W 1-2 |
| 12 | 15 | Haley ENGLE | Half Acre Cycling | 47:21. | 12:11. | 4 | W 1-2 |
| 13 | 7 | Ashley HEIDENREICH | Half Acre Cycling | 47:45. | 12:11. | 4 | W 1-2 |
| 14 | 18 | Lindsay KNIGHT | Tenspeed Hero | 48:15. | 12:55. | 4 | W 1-2 |
| 15 | 13 | Rebecca MIKRUT | Skunkworks Racing | 49:24. | 12:56. | 4 | W 1-2 |
| 16 | 51 | Amy SCHULTZ | L5 Racing | 49:42. | 12:19. | 4 | W 3-4 |
| 17 | 16 | Lauren WISCOMB | Bonkers Cycling | 50:46. | 14:07. | 4 | W 1-2 |
| 18 | 55 | Charlotte ERICKSON | Dirty Squirrels Racing | 50:54. | 12:58. | 4 | W 3-4 |
| 19 | 52 | Jennifer GROEN | Half Acre Cycling | 38:39. | 13:08. | 3 | W 3-4 |
| 20 | 54 | Laura ALAGNA | Union Cycling Team | 38:50. | 12:57. | 3 | W 3-4 |
| 21 | 66 | Allison ZMUDA | Comrade Cycles Racing | 39:02. | 13:21. | 3 | W 3-4 |
| 22 | 20 | Lindsay FREEBORN | PSIMET Racing | 39:17. | 13:21. | 3 | W 1-2 |
| 23 | 21 | Annicka CAMPBELL-DOLLAGHAN | Chicago Cuttin Crew | 39:51. | 13:31. | 3 | W 1-2 |
| 24 | 75 | Dianna RIEGER-ROBERTS | | 40:39. | 13:29. | 3 | W 3-4 |
| 25 | 57 | Melissa ZAMMIT | INTENT | 41:25. | 13:58. | 3 | W 3-4 |
| 26 | 53 | Jordan Gwyen AUSTIN | xXx Racing | 41:38. | 14:26. | 3 | W 3-4 |
| 27 | 59 | Olivia BEATY | | 41:47. | 13:32. | 3 | W 3-4 |
| 28 | 64 | Caitlin HAMER | Palomino American Cyclocross | 41:56. | 13:40. | 3 | W 3-4 |
| 29 | 58 | Laura SHIELDS | BFF Bikes | 42:44. | 14:41. | 3 | W 3-4 |
| 30 | 61 | Elizabeth FRANCZYK | Hollander Benelux Racing | 43:32. | 15:25. | 3 | W 3-4 |
| 31 | 70 | Gina JOHNSON | | 43:44. | 14:47. | 3 | W 3-4 |
| 32 | 63 | Carrie SCHOENEBERGER | Main Street Bicycles Sasquatch | 44:53. | 15:18. | 3 | W 3-4 |
| 33 | 68 | Anna HENSCHEL | Bonkers Cycling | 47:13. | 17:00. | 3 | W 3-4 |
| 34 | 72 | MaryAnn SEDOR | INTENT | 49:01. | 17:06. | 3 | W 3-4 |
| 35 | 76 | Morgan REX | | 51:51. | 17:35. | 3 | W 3-4 |
| DNF | 77 | Rachel PEARSON | | | 14:11. | 2 | W 3-4 |
| DNF | 62 | Katherine SCHAFER | PSIMET Racing | | 16:30. | 2 | W 3-4 |
| DNF | 60 | Meesa ROYSTER | Union Cycling Team | | 15:02. | 1 | W 3-4 |
