| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 889 | Bridget TOOLEY | Easton-Giant p/b Transitions Li | 40:06. | 6:52. | 6 | H100 |
| 2 | 890 | Kaia SCHMID | New England Devo p/b Cadence We | 40:34. | 6:50. | 6 | H100 |
| 3 | 893 | Eleanor DYAS | Blue Ridge Cross p/b JA King | 40:46. | 7:01. | 6 | H100 |
| 4 | 881 | Ava HOLMGREN | Hardwood Next Wave Cycling Team | 41:13. | 6:43. | 6 | H100 |
| 5 | 839 | Carrie MASTERS | Lionhearts Junior Racing | 41:13. | 6:44. | 6 | H100 |
| 6 | 882 | Isabella HOLMGREN | Hardwood Next Wave Cycling Team | 41:48. | 6:57. | 6 | H100 |
| 7 | 837 | Ella BRENNEMAN | CXHAIRS DEVO : TREK BIKES | 42:00. | 7:10. | 6 | H100 |
| 8 | 838 | Katherine SARKISOV | CXHairs Devo / TREK BIKES | 42:31. | 7:23. | 6 | H100 |
| 9 | 884 | Claire STECIUK | Hardwood Next Wave | 42:44. | 7:10. | 6 | H100 |
| 10 | 840 | Alexa STIERWALT | Aether Racing | 42:52. | 7:24. | 6 | H100 |
| 11 | 892 | Lauren ZOERNER | Alpha Bicycle Company Groove Su | 43:06. | 7:14. | 6 | H100 |
| 12 | 836 | Skylar BOVINE | CXHAIRS DEVO : TREK BIKES | 43:19. | 7:20. | 6 | H100 |
| 13 | 883 | Kelly LAWSON | Hardwood Next Wave | 43:25. | 7:17. | 6 | H100 |
| 14 | 841 | Dahlia KISSEL | Lionhearts Junior Racing | 43:54. | 7:33. | 6 | H100 |
| 15 | 861 | Ryley MOSHER | Wheel Works Racing | 44:24. | 7:32. | 6 | H100 |
| 16 | 842 | Brooklyn RADDIN | LOGIK | Junior Development Team | 45:49. | 7:41. | 6 | H100 |
| 17 | 844 | Jordan Gwyen AUSTIN | xXx racing Athletico | 46:50. | 8:06. | 6 | H100 |
| 18 | 846 | Emma BALDING | Midwest Devo | 39:00. | 7:56. | 5 | H100 |
| 19 | 848 | Lauren WEIGEL | Donkey Label Racing/ Stoemper | 40:27. | 8:32. | 5 | H100 |
| 20 | 849 | Audrey BAKER | Midwest Devo | 40:56. | 8:19. | 5 | H100 |
| 21 | 847 | Ragan WEIGEL | Donkey Label Racing/ Stoemper | 41:03. | 8:50. | 5 | H100 |
| 22 | 862 | Paula URBAN | Kenosha Kutters | 42:33. | 8:48. | 5 | H100 |
| 23 | 853 | Izabella SHAFFER | Summit freewheelers | 43:40. | 8:50. | 5 | H100 |
| 24 | 860 | Dakota Kennedy AUSTIN | xXx Racing-Athletico | 46:28. | 10:10. | 5 | H100 |
| DNF | 851 | Katie SMOCK | Midwest Devo | | 7:32. | 1 | H100 |
