| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 257 | Megan JASTRAB | Rally Sport Cycling Team | 44:07. | 44:07 |
| 2 | 245 | Ava SYKES | LUX/Sideshow p/b Specialized | 44:08. | s.t. |
| 3 | 244 | Elizabeth HARDEN | CWA Racing p/b TREK/CWA Racing | 44:09. | s.t. |
| 4 | 249 | Gracie PENDLETON | Nashville Local Cycling | 44:10. | s.t. |
| 5 | 242 | Summer GILBERT | Junior Cycle Sport/West Michiga | 44:10. | s.t. |
| 6 | 266 | Allison MCCURRY | Gray Goat Sports | 44:12. | 5 |
| 7 | 243 | Taylor JOHNSON | Melton Design/Cari Higgins RE C | 44:12. | s.t. |
| 8 | 264 | Caitrin HUYSMAN | | 44:14. | 7 |
| 9 | 247 | Gabrielle LEHNERT | LUX/Sideshow p/b Specialized | 44:16. | s.t. |
| 10 | 255 | Gabriella DIXON | Top Step Development | 44:17. | 10 |
| 11 | 268 | Julianna RUTECKI | Young Medalists | 44:20. | 13 |
| 12 | 256 | Vanessa ROMANO | Young Medalists | 44:20. | s.t. |
| 13 | 248 | Jane TULLIS | LUX/Sideshow p/b Specialized | 44:23. | 16 |
| 14 | 260 | Zoe TA-PEREZ | LUX/Sideshow p/b Specialized | 44:24. | 17 |
| 15 | 246 | Faith MONTREUIL | Gray Goat - Bullseye Total Medi | 44:42. | 35 |
| 16 | 258 | Maddie WOOLLEY | LUX Cycling | 45:05. | 58 |
| 17 | 261 | Katie CLOUSE | DNA Cycling Team/K4 Racing | 45:05. | s.t. |
| 18 | 265 | Abigail YATES | Matrix/RBM/Matrix Cycling Club | 45:07. | 1:00 |
| 19 | 263 | Rosalyn WILLEY | | 49:39. | 5:32 |
| 20 | 251 | Melissa GOMES | Quantum Racing Development pb S | 49:39. | s.t. |
| DNF | 253 | Petra SCHMIDTMANN | LUX Cycling | | |
| DNF | 254 | Cara KING | Edge Cycling | | |
| DNS | 241 | Lily ENSIGN | NorthStar Development Cycling | | |
| DNS | 250 | Renee MILLER | All American Bicycle Center Cyc | | |
| DNS | 259 | Kate SEILER | Sho-Air TWENTY20 Development | | |
| DNS | 267 | Leah WALTON | | | |
