| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 26 | Clara JAMES-HEER | Junior Cycle Sport/West Michiga | 46:43. | 46:43 |
| 2 | 27 | Alexa STIERWALT | Ignite Junior Racing/Cincinnati | 48:30. | 1:47 |
| 3 | 45 | Brooklyn RADDIN | TEAM LOGIK | 48:30. | s.t. |
| 4 | 36 | Dahlia KISSEL | Ignite Junior Racing/Cincinnati | 48:31. | s.t. |
| 5 | 38 | Homare YAMASHITA | Sho-Air TWENTY20 Devo | 48:31. | s.t. |
| 6 | 41 | Lilly MCLEOD | | 48:41. | 1:58 |
| 7 | 44 | Makala JARAMILLO | Rage Cycling Team | 49:04. | 2:21 |
| 8 | 37 | Katie SMOCK | Midwest Devo | 50:04. | 3:21 |
| 9 | 28 | Samantha SCOTT | Rock n Road Cyclery/Team Velosp | 50:05. | s.t. |
| 10 | 29 | Margot GROTLAND | Yokohama Racing p/b nine13sport | 51:04. | 4:21 |
| 11 | 42 | Emily WOOLFORD | New Mexico State University | 51:21. | 4:38 |
| 12 | 30 | Jorja BOND | Rage Cycling Team | 51:40. | 4:57 |
| 13 | 34 | Mackenzie MAYER | Duke University | 54:24. | 7:41 |
| 14 | 47 | Keira JONES | Oklahoma State University | 54:28. | 7:45 |
| 15 | 40 | Addie SLUTZ | Johns Hopkins University | 55:14. | 8:31 |
| 16 | 43 | Maize WIMBUSH | Sho-Air TWENTY20 Development | 55:22. | 8:39 |
| 17 | 31 | Azyra FRANKLIN | Getting It In Cyclists Inc | 56:35. | 9:52 |
| 18 | 46 | Anna WALTON | | 1:02:11. | 15:28 |
| DNS | 32 | Divya VERMA | Team T Town | | |
| @1Lap | 33 | Calla ANDERSON | CENLA STARS | | |
