| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 247 | Summer MOAK | Rally UHC Cycling | 50:05. | 50:05 |
| 2 | 260 | Samantha Ann SCHNIEDER | CWA Racing p/b TREK | 50:05. | s.t. |
| 3 | 182 | Lily WILLIAMS | Hagens Berman | Supermint | 50:06. | s.t. |
| 4 | 265 | Laura VAN GILDER | Fast Chance Womens Cycling p/b | 50:06. | s.t. |
| 5 | 230 | Madison KELLY | CWA Racing p/b TREK | 50:07. | 2 |
| 6 | 208 | Jennifer CAICEDO | Palmetto State Medical Cycling | 50:07. | s.t. |
| 7 | 219 | Daniely DEL VALLE GARCIA | CWA Racing p/b TREK | 50:08. | s.t. |
| 8 | 203 | Madeline BEMIS | Milligan Cycling | 50:08. | s.t. |
| 9 | 243 | Ayesha MCGOWAN | A Quick Brown Fox p/b Liv Racin | 50:08. | s.t. |
| 10 | 216 | Alexi COSTA | Pursuit Cycing | 50:08. | s.t. |
| 11 | 211 | Ana Teresa CASAS | CWA Racing p/b TREK | 50:08. | s.t. |
| 12 | 240 | Courteney LOWE | Fast Chance Womens Cycling p/b | 50:09. | s.t. |
| 13 | 210 | Erica CARNEY | Rally UHC Cycling | 50:09. | s.t. |
| 14 | 200 | Pip SUTTON | Specialized Wolfpack p/b Jakroo | 50:09. | s.t. |
| 15 | 252 | Tina PIC | Colavita/Bialetti Pro Cycling | 50:09. | s.t. |
| 16 | 217 | Sommers CREED | 706 Project / United Healthcare | 50:10. | s.t. |
| 17 | 236 | Kathleen LAPORTE | L5 Flyers | 50:11. | 6 |
| 18 | 253 | Caroline POOLE | Specialized Wolfpack p/b Jakroo | 50:13. | 8 |
| 19 | 181 | Elizabeth HARDEN | CWA Racing p/b TREK | 50:48. | 43 |
| 20 | 223 | Natalia FRANCO VILLEGAS | Colavita/Bialetti Pro Cycling T | 50:54. | 49 |
| @1Lap | 205 | Vanessa BOTERO | Milligan Cycling | | |
| @1Lap | 184 | Gracie PENDLETON | Milligan College | | |
| @2Lap | 185 | Meade PLUM | Milligan College | | |
| @2Lap | 261 | Yussely SOTO | Colavita/Bialetti Pro Cycling | | |
