| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 124 | Melissa BARKER | Feedback Sports Racing | 41:20. | 8:29. | 5 | F 40-44 123 |
| 2 | 121 | Monica LLOYD | Olympia Orthopaedic Associates | 41:41. | 8:27. | 5 | F 40-44 123 |
| 3 | 125 | Bonnie SYMES | Campus WheelWorks | 42:21. | 8:33. | 5 | F 40-44 123 |
| 4 | 123 | Melanie CARTER | Feedback Sports Racing | 43:32. | 8:46. | 5 | F 40-44 123 |
| 5 | 134 | Kenda SUPER | Hagens Berman Cycling | 43:51. | 8:20. | 5 | F 40-44 123 |
| 6 | 142 | Joanne GROGAN | BMB Racing | 44:17. | 9:07. | 5 | F 40-44 123 |
| 7 | 120 | Jennifer PARK | Bicycle Heaven / PVA | 45:06. | 8:44. | 5 | F 40-44 123 |
| 8 | 129 | Abigail WALKER | | 45:20. | 9:03. | 5 | F 40-44 123 |
| 9 | 127 | Nikki BALDERSON | | 45:33. | 8:51. | 5 | F 40-44 123 |
| 10 | 126 | Evie EDWARDS | Feed Hungry Kids Project p/b Ha | 45:34. | 8:52. | 5 | F 40-44 123 |
| 11 | 143 | Alicia PRUSINSKI | | 47:13. | 9:34. | 5 | F 40-44 123 |
| 12 | 132 | Maria WEESE | Ghisallo Cycling Team Inc. | 48:25. | 10:14. | 5 | F 40-44 123 |
| 13 | 137 | Sara KATZ | Gus Cycling | 49:14. | 10:15. | 5 | F 40-44 123 |
| 14 | 144 | Dawn HILL | L5Flyers Cycling Team | 39:37. | 10:05. | 4 | F 40-44 123 |
| 15 | 139 | Stephanie SWAN | PATHFINDER of WV Race Team | 40:01. | 10:02. | 4 | F 40-44 123 |
| 16 | 147 | Aerah HARDIN | SHAM EVENTS | 40:50. | 10:20. | 4 | F 40-44 123 |
| 17 | 128 | Melissa PIERAGOSTINI | Twisted Cog Racing p/b Stable 1 | 42:07. | 10:15. | 4 | F 40-44 123 |
| 18 | 141 | Amanda BYE | Naked Womens Racing | 43:09. | 11:01. | 4 | F 40-44 123 |
| 19 | 136 | Elizabeth SCHLAUDECKER | Team Hungry | 43:30. | 10:51. | 4 | F 40-44 123 |
| 20 | 130 | Tara SEPLAVY | Foxy Moxy Dev | 45:00. | 11:58. | 4 | F 40-44 123 |
| 21 | 133 | Korin EVANS | | 46:51. | 12:28. | 4 | F 40-44 123 |
| 22 | 131 | Kelly KOWALCZYK | | 47:47. | 11:21. | 4 | F 40-44 123 |
| 23 | 122 | Amanda MCNABB | Team Thrive p/b Kaiser Permanen | 47:55. | 11:48. | 4 | F 40-44 123 |
| 24 | 145 | Kris TILES | Gryphon Velo Racing | 39:04. | 16:03. | 3 | F 40-44 123 |
