| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 2 | Maria LARKIN | The Call Up pb Bike Law | 43:57. | 6:42. | 6 | W 1-2 |
| 2 | 1 | Katherine ISERMANN | PSIMET Racing | 43:57. | 6:42. | 6 | W 1-2 |
| 3 | 3 | Sydney GUAGLIARDO | | 44:05. | 6:50. | 6 | W 1-2 |
| 4 | 5 | Alicia PRUSINSKI | MOX Multisport | 45:44. | 7:22. | 6 | W 1-2 |
| 5 | 4 | Amanda SCHAAP | Speed Merchants | 46:54. | 7:32. | 6 | W 1-2 |
| 6 | 8 | Ashley HEIDENREICH | Half Acre Cycling | 47:03. | 7:28. | 6 | W 1-2 |
| 7 | 11 | Rebecca MIKRUT | Spidermonkey Cycling | 47:12. | 7:26. | 6 | W 1-2 |
| 8 | 12 | Lindsay KNIGHT | Tenspeed Hero | 47:30. | 7:37. | 6 | W 1-2 |
| 9 | 6 | Kristen HOSEY | UPB Cycling | 48:12. | 8:11. | 6 | W 1-2 |
| 10 | 70 | Haley ENGLE | Half Acre Cycling | 48:19. | 7:35. | 6 | W 3-4 |
| 11 | 52 | May TSUPROS | Comrade Cycles Racing | 48:53. | 7:32. | 6 | W 3-4 |
| 12 | 10 | Jodie PRESTINE | Velo Femmes Racing | 49:01. | 7:55. | 6 | W 1-2 |
| 13 | 59 | Emma OSBORNE | Team Extreme | 49:03. | 7:21. | 6 | W 3-4 |
| 14 | 46 | Ashley MOORE | Spidermonkey Cycling | 49:44. | 7:50. | 6 | W 3-4 |
| 15 | 66 | Jennifer GROEN | Half Acre Cycling | 50:08. | 7:51. | 6 | W 3-4 |
| 16 | 47 | Lorraine MCCARTHY | Force Power Racing | 42:31. | 7:52. | 5 | W 3-4 |
| 17 | 72 | Beth CHRISTIANSEN | Union Cycling Team | 42:47. | 8:02. | 5 | W 3-4 |
| 18 | 68 | Elizabeth FRANCZYK | Palomino American Cyclocross | 42:50. | 7:59. | 5 | W 3-4 |
| 19 | 13 | Lauren WISSMAN | | 42:55. | 8:20. | 5 | W 1-2 |
| 20 | 69 | Charlotte ERICKSON | Dirty Squirrels Racing | 43:12. | 7:52. | 5 | W 3-4 |
| 21 | 62 | Lauren JACOBS | xXx Racing | 43:13. | 7:52. | 5 | W 3-4 |
| 22 | 49 | Sam SCIPIO | Comrade Cycles Racing | 43:21. | 8:09. | 5 | W 3-4 |
| 23 | 73 | Kristina CAUDLE | BFF Bikes | 43:50. | 8:18. | 5 | W 3-4 |
| 24 | 53 | Megan SYBELDON | Conemaugh Women???s Racing | 43:54. | 8:06. | 5 | W 3-4 |
| 25 | 57 | Arianna SCHAFER | PSIMET Racing | 44:03. | 7:48. | 5 | W 3-4 |
| 26 | 75 | Laura ALAGNA | Union Cycling Team | 44:53. | 8:30. | 5 | W 3-4 |
| 27 | 58 | Dianna RIEGER-ROBERTS | | 45:02. | 8:26. | 5 | W 3-4 |
| 28 | 60 | Jennifer MOSLEY | Half Acre Cycling | 45:26. | 8:24. | 5 | W 3-4 |
| 29 | 56 | Katherine SCHAFER | PSIMET Racing | 45:57. | 8:37. | 5 | W 3-4 |
| 30 | 67 | Theia FRIESTEDT | Velosmith Bicycle Studio | 46:12. | 8:25. | 5 | W 3-4 |
| 31 | 54 | Laura SHIELDS | BFF Bikes | 46:52. | 8:37. | 5 | W 3-4 |
| 32 | 74 | Olivia BEATY | Union Cycling Team | 47:23. | 8:49. | 5 | W 3-4 |
| 33 | 71 | Lydia ELBERT | The Pony Shop | 48:06. | 9:09. | 5 | W 3-4 |
| 34 | 48 | Lauren MATRICARDI | Spidermonkey Cycling | 49:25. | 10:01. | 5 | W 3-4 |
| 35 | 61 | Elizabeth MCKINLEY | Comrade Cycles Racing | 49:41. | 9:45. | 5 | W 3-4 |
| 36 | 64 | Jessica HELMLINGER | Main Street Bicycles Sasquatch | 49:53. | 9:08. | 5 | W 3-4 |
| 37 | 55 | Carrie SCHOENEBERGER | Main Street Bicycles Sasquatch | 49:59. | 8:57. | 5 | W 3-4 |
| 38 | 51 | Michele UNDERWOOD | MOX Multisport | 51:35. | 9:47. | 5 | W 3-4 |
| 39 | 65 | Emily HEAVEY | Half Acre Cycling | 46:10. | 11:15. | 4 | W 3-4 |
| DNS | 9 | Jessica WHITON | xXx Racing | | | 0 | W 1-2 |
| DNS | 63 | Anna HENSCHEL | Bonkers Cycling | | | 0 | W 3-4 |
