| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 528 | Beth CATHERWOOD | US Military Endurance Sports | 44:08. | 10:09. | 4 | W 4-5 |
| 2 | 525 | Sarah GRANADE | VeloWorks-Spokes Etc | 44:11. | 10:14. | 4 | W 4-5 |
| 3 | 530 | Johanna NEUBER | Rogue Velo Racing | 44:46. | 10:37. | 4 | W 4-5 |
| 4 | 540 | Mary STILL | Rogue Velo Racing | 35:55. | 10:52. | 3 | W 4-5 |
| 5 | 509 | Jessie PARKER | Baltimore Bicycle Club/Team BBC | 35:55. | 11:18. | 3 | W 4-5 |
| 6 | 522 | Chloe TRAN | Baltimore Youth Cycling | 36:24. | 11:21. | 3 | W 4-5 |
| 7 | 515 | Kathleen HOLMES | Unattached | 36:31. | 10:59. | 3 | W 4-5 |
| 8 | 518 | Miriam JONES | Team Bikenetic | 36:39. | 11:15. | 3 | W 4-5 |
| 9 | 545 | Deborah SHEARER | Unattached | 36:48. | 11:08. | 3 | W 4-5 |
| 10 | 524 | Irina SARKISOV | Unattached | 37:12. | 11:31. | 3 | W 4-5 |
| 11 | 559 | Leah FANTLE | Unattached | 37:20. | 11:45. | 3 | W 4-5 |
| 12 | 507 | Melissa KELLSTROM | National Capital Velo Club/Unit | 37:21. | 11:37. | 3 | W 4-5 |
| 13 | 550 | Christy LAUSCH | Unattached | 37:22. | 11:38. | 3 | W 4-5 |
| 14 | 552 | Rose BYLER | The Bike Rack/Bike Rack Racing | 37:45. | 11:28. | 3 | W 4-5 |
| 15 | 504 | Kelly ROBERSON | Unattached | 37:49. | 11:35. | 3 | W 4-5 |
| 16 | 520 | Lindsay OCONNOR | Unattached | 38:07. | 11:43. | 3 | W 4-5 |
| 17 | 526 | Lindsay NELSON | Twin Six Team Metal | 38:48. | 11:48. | 3 | W 4-5 |
| 18 | 527 | Alexis ROHDE | Baltimore Bicycle Club/Team BBC | 39:05. | 11:55. | 3 | W 4-5 |
| 19 | 548 | Natasha KOERMER | Pro Bike FC | 39:06. | 12:24. | 3 | W 4-5 |
| 20 | 553 | Alison MCKEITHAN | Unattached | 39:19. | 11:40. | 3 | W 4-5 |
| 21 | 511 | Ana BROYLES | Unattached | 39:36. | 13:09. | 3 | W 4-5 |
| 22 | 534 | Downing LU | Rock Creek Velo | 39:42. | 12:09. | 3 | W 4-5 |
| 23 | 539 | Andrea DOBBINS | Unattached | 39:56. | 12:03. | 3 | W 4-5 |
| 24 | 506 | Maureen BOYLE | Team Bikenetic | 39:58. | 12:37. | 3 | W 4-5 |
| 25 | 555 | Rebecca AUYER | Unattached | 40:04. | 11:53. | 3 | W 4-5 |
| 26 | 505 | Jocelyn HERRINGTON | Team Bikenetic | 40:15. | 12:30. | 3 | W 4-5 |
| 27 | 503 | Christine FELDT | Team Bikenetic | 40:39. | 12:29. | 3 | W 4-5 |
| 28 | 537 | Rebecca IRWIN KENNEDY | Rock Creek Velo | 40:40. | 12:28. | 3 | W 4-5 |
| 29 | 557 | Sharon SQUASSONI | Unattached | 40:55. | 12:37. | 3 | W 4-5 |
| 30 | 549 | April WILLIAMSON | The Bike Rack/Bike Rack Racing | 41:11. | 12:52. | 3 | W 4-5 |
| 31 | 558 | Virginia LINGHAM | Vanderkitten VIP | 41:22. | 12:33. | 3 | W 4-5 |
| 32 | 556 | Dorothy MOZLOOM | Unattached | 41:51. | 12:52. | 3 | W 4-5 |
| 33 | 544 | Raelene MENESES | Unattached | 41:55. | 12:42. | 3 | W 4-5 |
| 34 | 523 | Jennifer WERNER | Unattached | 41:59. | 13:03. | 3 | W 4-5 |
| 35 | 543 | Devan TRACY | Unattached | 42:44. | 13:02. | 3 | W 4-5 |
| 36 | 514 | Patricia LEDESMA | Colavita Racing Inc. | 42:45. | 13:47. | 3 | W 4-5 |
| 37 | 554 | Melissa LUKASIEWICZ | Unattached | 43:29. | 13:11. | 3 | W 4-5 |
| 38 | 531 | Julie SIMS | Unattached | 44:54. | 14:10. | 3 | W 4-5 |
| 39 | 535 | Sara MCGAUGHEY | Unattached | 46:16. | 14:27. | 3 | W 4-5 |
| 40 | 536 | Evelyn MURCIA | Phoenix Bikes/Phoenix Bikes | 46:34. | 14:46. | 3 | W 4-5 |
| 41 | 541 | Anna POWELL | Altius Cycling Team | 47:45. | 15:06. | 3 | W 4-5 |
| 42 | 510 | Lisa MAY | Unattached | 35:33. | 16:08. | 2 | W 4-5 |
| 43 | 529 | Rachel KRISTY | Team Sticky Fingers | 35:47. | 16:40. | 2 | W 4-5 |
| 44 | 517 | Jennifer STACK | Lateral Stress Velo Inc. | 36:03. | 16:51. | 2 | W 4-5 |
| 45 | 542 | Margaret KURTTS | Unattached | 37:22. | 18:12. | 2 | W 4-5 |
| 46 | 546 | Renada SNODGRASS | RC Racing | 37:24. | 17:53. | 2 | W 4-5 |
| 47 | 538 | Shannon GREENE | Unattached | 38:38. | 16:38. | 2 | W 4-5 |
| DNF | 532 | Natalie TANG | Unattached | | 15:27. | 2 | W 4-5 |
