| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 538 | Julie HUANG TUCKER | Pro Bike FC Cycling Team | 44:24. | 10:16. | 4 | W 4-5 |
| 2 | 540 | Sabine BUCCHERI | Baltimore Youth Cycling | 44:35. | 10:17. | 4 | W 4-5 |
| 3 | 536 | Johanna NEUBER | Rogue Velo Racing | 44:41. | 10:14. | 4 | W 4-5 |
| 4 | 525 | Robin MILLICAN | Unattached | 45:02. | 10:30. | 4 | W 4-5 |
| 5 | 528 | Beth CATHERWOOD | US Military Endurance Sports | 45:22. | 10:50. | 4 | W 4-5 |
| 6 | 530 | Sarah GRANADE | VeloWorks-Spokes Etc | 45:45. | 10:38. | 4 | W 4-5 |
| 7 | 547 | Mary STILL | Rogue Velo Racing | 46:06. | 10:35. | 4 | W 4-5 |
| 8 | 553 | Deborah SHEARER | Unattached | 47:40. | 10:57. | 4 | W 4-5 |
| 9 | 534 | Edrie ORTEGA | Unattached | 48:11. | 11:29. | 4 | W 4-5 |
| 10 | 508 | Jessie PARKER | Baltimore Bicycle Club/Team BBC | 48:17. | 11:28. | 4 | W 4-5 |
| 11 | 527 | Irina SARKISOV | Unattached | 48:17. | 11:20. | 4 | W 4-5 |
| 12 | 546 | Jessica CONLEY | Unattached | 48:25. | 11:12. | 4 | W 4-5 |
| 13 | 549 | Chelsea RICHWINE | VeloWorks-Spokes Etc | 51:50. | 13:11. | 4 | W 4-5 |
| 14 | 521 | Lindsay OCONNOR | Unattached | 38:21. | 12:00. | 3 | W 4-5 |
| 15 | 509 | Ashlea GRAY | Baltimore Bicycle Club/Team BBC | 38:30. | 11:50. | 3 | W 4-5 |
| 16 | 507 | Melissa KELLSTROM | National Capital Velo Club/Unit | 38:34. | 12:00. | 3 | W 4-5 |
| 17 | 504 | Kelly ROBERSON | Unattached | 38:38. | 11:51. | 3 | W 4-5 |
| 18 | 545 | Andrea DOBBINS | Unattached | 38:42. | 11:52. | 3 | W 4-5 |
| 19 | 505 | Jocelyn HERRINGTON | Team Bikenetic | 39:14. | 12:03. | 3 | W 4-5 |
| 20 | 503 | Christine FELDT | Team Bikenetic | 39:22. | 12:08. | 3 | W 4-5 |
| 21 | 554 | Em CHIU | Pro Bike FC Cycling Team | 39:39. | 12:09. | 3 | W 4-5 |
| 22 | 513 | Ana BROYLES | Unattached | 39:40. | 12:30. | 3 | W 4-5 |
| 23 | 558 | Alison MCKEITHAN | Unattached | 39:51. | 12:47. | 3 | W 4-5 |
| 24 | 556 | Rhonda MCLAUGHLIN | Squadra Coppi | 39:59. | 12:40. | 3 | W 4-5 |
| 25 | 548 | Julia GLOWACKI | Evolution Cycling Club p/b Long | 40:26. | 12:44. | 3 | W 4-5 |
| 26 | 533 | Rebecca AUYER | Unattached | 40:38. | 12:31. | 3 | W 4-5 |
| 27 | 518 | Sonja RODMAN | Unattached | 40:47. | 12:44. | 3 | W 4-5 |
| 28 | 510 | Sara LAPPANO | Team Sticky Fingers | 41:25. | 13:15. | 3 | W 4-5 |
| 29 | 551 | Raelene MENESES | Unattached | 41:45. | 12:58. | 3 | W 4-5 |
| 30 | 542 | Heather PROCHNOW | Unattached | 42:31. | 12:59. | 3 | W 4-5 |
| 31 | 516 | Patricia LEDESMA | Colavita Racing Inc. | 42:55. | 13:24. | 3 | W 4-5 |
| 32 | 555 | Briana DENNEY | Century Road Club Assoc/Houliha | 43:39. | 13:00. | 3 | W 4-5 |
| 33 | 526 | Jennifer WERNER | Unattached | 43:50. | 13:36. | 3 | W 4-5 |
| 34 | 531 | Rachel MELO | Team-Not-Team | 44:09. | 14:00. | 3 | W 4-5 |
| 35 | 524 | Kelley MACEWEN | The Bike Rack/Bike Rack Racing | 44:13. | 13:59. | 3 | W 4-5 |
| 36 | 557 | Melissa LUKASIEWICZ | Unattached | 44:57. | 13:25. | 3 | W 4-5 |
| 37 | 537 | Sheila MAHADEVAN | Unattached | 46:18. | 14:33. | 3 | W 4-5 |
| 38 | 539 | Julie SIMS | Unattached | 47:37. | 14:27. | 3 | W 4-5 |
| 39 | 523 | Sarah RENS | Banana Pancakes | 48:36. | 14:28. | 3 | W 4-5 |
| 40 | 535 | Kim DACOSTA-AZAR | Unattached | 48:41. | 15:23. | 3 | W 4-5 |
| 41 | 532 | Sheila HOURIHAN | Baltimore Youth Cycling | 51:43. | 15:53. | 3 | W 4-5 |
| 42 | 552 | Karen RACOWSKY | Unattached | 51:55. | 16:26. | 3 | W 4-5 |
| 43 | 519 | Jennifer STACK | Lateral Stress Velo Inc. | 52:36. | 17:27. | 3 | W 4-5 |
| 44 | 512 | Lisa MAY | Unattached | 52:37. | 16:56. | 3 | W 4-5 |
| 45 | 529 | Riley BARTLEBAUGH | Unattached | 53:45. | 16:58. | 3 | W 4-5 |
| 46 | 550 | Robyn SPANN | Team Bikenetic | 43:05. | 21:06. | 2 | W 4-5 |
| 47 | 559 | Cathryn MURPHEY | Unattached | 15:40. | 15:40. | 1 | W 4-5 |
| 48 | 544 | Cameron OLIVER | National Capital Velo Club/Unit | 17:22. | 17:22. | 1 | W 4-5 |
| 49 | 541 | Laura BENNETT | Pro Bike FC Cycling Team | 21:45. | 21:45. | 1 | W 4-5 |
| DNF | 543 | Lauren JENKINS | VeloWorks-Spokes Etc | | 15:18. | 1 | W 4-5 |
