| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 576 | Mary COCHRAN | Unattached | 40:14. | 9:57. | 4 | Mas W 40 |
| 2 | 577 | Jennifer KRAUT | Colavita Racing Inc. | 41:22. | 10:06. | 4 | Mas W 40 |
| 3 | 578 | Traci RODOSTA | Unattached | 41:59. | 10:30. | 4 | Mas W 40 |
| 4 | 580 | Deb Sweeney WHITMORE | Crosswind Sports Management/CTS | 42:36. | 10:37. | 4 | Mas W 40 |
| 5 | 591 | Mary BREED | National Capital Velo Club/Unit | 44:14. | 10:29. | 4 | Mas W 40 |
| 6 | 581 | Julia SLEJKO | Charm City Cycling LLC | 44:44. | 11:10. | 4 | Mas W 40 |
| 7 | 579 | Elizabeth HARLOW | Unattached | 45:13. | 11:13. | 4 | Mas W 40 |
| 8 | 590 | Korin EVANS | Unattached | 46:16. | 11:57. | 4 | Mas W 40 |
| 9 | 583 | Cary COSPER | Unattached | 46:26. | 11:19. | 4 | Mas W 40 |
| 10 | 585 | Laura PYLE | BFF Bikes | 47:37. | 11:43. | 4 | Mas W 40 |
| 11 | 586 | Rosemarie BEALE | Rock Creek Velo | 48:11. | 11:51. | 4 | Mas W 40 |
| 12 | 589 | Jennifer EVERHARD | Unattached | 48:34. | 11:54. | 4 | Mas W 40 |
| 13 | 588 | Heather HEINRICH | Unattached | 49:42. | 11:59. | 4 | Mas W 40 |
| 14 | 587 | Sonya RICHESON | Unattached | 49:55. | 12:35. | 4 | Mas W 40 |
| 15 | 582 | Adrien ARNOLD | River CIty Womens Racing | 38:36. | 12:45. | 3 | Mas W 40 |
| 16 | 594 | Sharon SQUASSONI | Unattached | 38:57. | 12:46. | 3 | Mas W 40 |
| 17 | 593 | Anna CLARK | Pro Bike FC Cycling Team | 42:39. | 13:42. | 3 | Mas W 40 |
| 18 | 592 | Monika KORNHAUSER | National Capital Velo Club/Unit | 44:45. | 14:45. | 3 | Mas W 40 |
