| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 58 | Ella BRENNEMAN | TREK BIKES | 38:42. | 9:28. | 4 | W 2-3 |
| 2 | 59 | Katherine SARKISOV | TREK BIKES | 38:54. | 9:39. | 4 | W 2-3 |
| 3 | 63 | Erin BOUGIE | Catoctin Cycling Club/NSMI RACI | 40:00. | 10:05. | 4 | W 2-3 |
| 4 | 78 | Jen HOCK | VeloDash | 40:00. | 9:46. | 4 | W 2-3 |
| 5 | 65 | Holly NECKERMANN | Unattached | 40:03. | 10:08. | 4 | W 2-3 |
| 6 | 68 | Erin MASCELLI | Yukato Yoga p/b Two Rivers Brew | 40:04. | 10:06. | 4 | W 2-3 |
| 7 | 64 | Carley LEINS | Unattached | 40:15. | 9:55. | 4 | W 2-3 |
| 8 | 77 | Lindsey CRIFASI | Team Handmade | 40:25. | 10:07. | 4 | W 2-3 |
| 9 | 61 | Sabrina HAYES | Bear Development Team | 40:28. | 10:07. | 4 | W 2-3 |
| 10 | 69 | Victoria STEEN | Lady Gnar Shredders | 40:45. | 10:19. | 4 | W 2-3 |
| 11 | 60 | Shane FERRO | Century Road Club Assoc/CityMD | 40:49. | 10:23. | 4 | W 2-3 |
| 12 | 73 | Jenna LATOURNICHOLS | Pro Bike FC Cycling Team | 40:51. | 10:14. | 4 | W 2-3 |
| 13 | 80 | Elise ROWE | La Prima Espresso Co. Racing | 41:30. | 10:35. | 4 | W 2-3 |
| 14 | 79 | Christina HOSENFELD | Bluemont Connection | 41:37. | 10:17. | 4 | W 2-3 |
| 15 | 74 | Lucia DENG | Unattached | 41:45. | 10:35. | 4 | W 2-3 |
| 16 | 66 | Eugena GUNDERSON | 717 Cycling club | 41:54. | 9:56. | 4 | W 2-3 |
| 17 | 75 | Olivia SANDOVAL | Baltimore Youth Cycling | 41:54. | 10:25. | 4 | W 2-3 |
| 18 | 84 | Tori RIEMERSMA | White Mountain Road Club/Landis | 41:58. | 10:42. | 4 | W 2-3 |
| 19 | 71 | Amanda GRAVENHORST | The Bike Rack/Bike Rack Racing | 41:59. | 10:15. | 4 | W 2-3 |
| 20 | 70 | Natasha CALDERWOOD | The Bike Rack/Bike Rack Racing | 42:11. | 10:29. | 4 | W 2-3 |
| 21 | 94 | Laura MCMILLION | Charlottesville Racing Club/Blu | 42:41. | 10:46. | 4 | W 2-3 |
| 22 | 81 | Melissa SEEK | Celerity Cycling/SEAVS/Haymarke | 42:51. | 11:30. | 4 | W 2-3 |
| 23 | 82 | Jenny BOURQUE | Bluemont Connection | 43:12. | 11:05. | 4 | W 2-3 |
| 24 | 89 | Kathryn CAMPBELL | VeloDash | 43:45. | 10:42. | 4 | W 2-3 |
| 25 | 87 | Lisa YOUNG | National Capital Velo Club/Unit | 43:52. | 10:51. | 4 | W 2-3 |
| 26 | 62 | Emma FREYMANN | Laser Cats Feline All Stars | 43:53. | 11:15. | 4 | W 2-3 |
| 27 | 95 | Andrea ARCHER | Century Road Club Assoc/Dannys | 44:07. | 10:55. | 4 | W 2-3 |
| 28 | 93 | Greta DANIELS | J Mac Cycling LLC | 44:14. | 10:57. | 4 | W 2-3 |
| 29 | 67 | Holli FINNEREN | Unattached | 44:48. | 11:15. | 4 | W 2-3 |
| 30 | 72 | Cassie BOPF | Rock Creek Velo | 45:03. | 11:43. | 4 | W 2-3 |
| 31 | 86 | Caryn WILLIS | La Prima Espresso Co. Racing | 46:16. | 11:48. | 4 | W 2-3 |
| 32 | 85 | Laura NORRIS | Baltimore Bicycle Club/Team BBC | 46:48. | 11:43. | 4 | W 2-3 |
| 33 | 91 | Rosalind HAIN | Colavita Racing Inc. | 47:23. | 11:54. | 4 | W 2-3 |
| 34 | 92 | Amanda GILBERT | Unattached | 47:56. | 12:08. | 4 | W 2-3 |
| 35 | 90 | Lauren PETERSON | National Capital Velo Club/Unit | 48:45. | 12:32. | 4 | W 2-3 |
| 36 | 76 | Tara SEPLAVY | Danger Diamond/Foxy Moxy Dev | 50:12. | 12:53. | 4 | W 2-3 |
