| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 103 | Britt MASON | Battley Womens Road Team | 40:21. | 10:54. | 4 | W 2-3 |
| 2 | 105 | Ella BRENNEMAN | CXHairs Devo | 41:16. | 11:08. | 4 | W 2-3 |
| 3 | 104 | Jen TILLMAN | Joes Bike Shop Racing Team | 41:50. | 11:32. | 4 | W 2-3 |
| 4 | 102 | Grace MATTERN | Park Ave Bike p/b Borah Teamwea | 42:42. | 11:38. | 4 | W 2-3 |
| 5 | 101 | Skylar BOVINE | Happy Tooth Womens Racing | 43:12. | 11:43. | 4 | W 2-3 |
| 6 | 107 | Emma FREYMANN | Laser Cats Feline Allstars | 43:45. | 12:00. | 4 | W 2-3 |
| 7 | 110 | Katherine SARKISOV | CXHairs Devo | 43:49. | 11:45. | 4 | W 2-3 |
| 8 | 112 | Caitlin THOMPSON | Team Laser Cats | 43:56. | 12:03. | 4 | W 2-3 |
| 9 | 135 | Sabrina HAYES | SEAVS / Haymarket Bicycles | 44:33. | 12:13. | 4 | W 2-3 |
| 10 | 119 | Shane FERRO | CRCA/CityMD Womens Racing | 44:36. | 12:28. | 4 | W 2-3 |
| 11 | 106 | Erin BOUGIE | NSMI Racing | 45:10. | 12:41. | 4 | W 2-3 |
| 12 | 137 | Holly NECKERMANN | No. 22 Racing | 45:11. | 12:07. | 4 | W 2-3 |
| 13 | 109 | Emily WOOLFORD | Baltimore Youth Cycling | 45:46. | 12:46. | 4 | W 2-3 |
| 14 | 118 | Stephanie THOMPSON | CRCA/Rockstar Games | 45:58. | 12:33. | 4 | W 2-3 |
| 15 | 128 | Elise ROWE | La Prima Espresso Co. Racing | 46:28. | 12:36. | 4 | W 2-3 |
| 16 | 136 | Christina HOSENFELD | Bluemont Connection | 47:16. | 13:22. | 4 | W 2-3 |
| 17 | 117 | Vanessa ROMANO | Young Medalists | 47:23. | 12:46. | 4 | W 2-3 |
| 18 | 114 | Olivia SANDOVAL | Baltimore Youth Cycling | 47:29. | 13:36. | 4 | W 2-3 |
| 19 | 123 | Jessica STUART | No.22 Racing | 47:58. | 12:47. | 4 | W 2-3 |
| 20 | 115 | Natasha CALDERWOOD | The Bike Rack/Bike Rack Racing | 48:14. | 13:04. | 4 | W 2-3 |
| 21 | 122 | Jenny BOURQUE | Bluemont Connection | 48:48. | 13:25. | 4 | W 2-3 |
| 22 | 131 | Kelly LYNAM | JAMSQUAD Cycling | 48:50. | 13:34. | 4 | W 2-3 |
| 23 | 126 | Andrea ARCHER | CRCA/Houlihan Lokey | 49:01. | 13:29. | 4 | W 2-3 |
| 24 | 124 | Krista SILVERS | Rogue velo racing | 49:11. | 13:12. | 4 | W 2-3 |
| 25 | 129 | Kate CAMPBELL | VeloDash | 49:13. | 13:33. | 4 | W 2-3 |
| 26 | 134 | Greta DANIELS | JMac Cycling | 49:14. | 13:10. | 4 | W 2-3 |
| 27 | 127 | Laura MCMILLION | Blue Ridge Cyclery Racing | 49:44. | 14:04. | 4 | W 2-3 |
| 28 | 121 | Bethany NEWTON | RACE CF | 50:46. | 13:45. | 4 | W 2-3 |
| 29 | 132 | Carla CLIFFORD | KING KOG / SUN AND AIR | 53:12. | 14:55. | 4 | W 2-3 |
| 30 | 133 | Lauren PETERSON | NCVC/UnitedHealthcare | 40:05. | 15:11. | 3 | W 2-3 |
| DNF | 120 | Rosalind PATTERSON | Rare Disease Cycling / Keswick | | 14:44. | 2 | W 2-3 |
| DNF | 116 | Lucia DENG | No.22 Racing | | 10:33. | 1 | W 2-3 |
