| Rank | Bib | Name | Affiliation | Time | Split Time | Laps |
| 1 | 310 | Christina HOSENFELD | Bluemont Connection | 45:12. | 12:28. | 4 |
| 2 | 336 | Johanna NEUBER | | 47:24. | 13:17. | 4 |
| 3 | 322 | Melissa PAQUETTE | QCW Cycling p.b. Breakawaybikes | 47:42. | 13:20. | 4 |
| 4 | 303 | Sabine BUCCHERI | Baltimore Youth Cycling | 47:56. | 13:09. | 4 |
| 5 | 321 | Clem NIXON | KING KOG/ SUN AND AIR | 48:14. | 13:18. | 4 |
| 6 | 333 | Beth CATHERWOOD | US Military Endurance Sports | 49:00. | 13:29. | 4 |
| 7 | 325 | Sarah GRANADE | VeloWorks-Spokes Etc | 51:15. | 13:52. | 4 |
| 8 | 323 | Mary STILL | Rogue Velo Racing | 51:41. | 13:55. | 4 |
| 9 | 324 | Caryn WILLIS | La Prima Espresso Racing | 51:47. | 14:15. | 4 |
| 10 | 315 | Sara RUDOLPH | Ride Brooklyn | 51:57. | 14:05. | 4 |
| 11 | 311 | Ashlea GRAY | Team BBC p/b Baltimore Bicycle | 52:07. | 14:28. | 4 |
| 12 | 326 | Irina SARKISOV | | 52:50. | 14:23. | 4 |
| 13 | 342 | Christy LAUSCH | Mini Cow Tri | 53:25. | 15:44. | 4 |
| 14 | 308 | Kelly ROBERSON | Great Eskape | 39:23. | 15:18. | 3 |
| 15 | 346 | ROSS SARAH | DAY OF REG | 39:38. | 15:09. | 3 |
| 16 | 331 | Lindsay OCONNOR | SEAVS/Haymarket | 39:42. | 14:59. | 3 |
| 17 | 304 | Melissa KELLSTROM | NCVC/UnitedHeathcare | 39:44. | 15:48. | 3 |
| 18 | 309 | Jocelyn HERRINGTON | Team Bikenetic | 40:01. | 14:23. | 3 |
| 19 | 332 | Kathryn HURCHLA | VeloDash | 41:00. | 15:25. | 3 |
| 20 | 334 | Mary FANELLI LUND | Sturdy Girl/Vanderkitten | 41:27. | 15:50. | 3 |
| 21 | 329 | Raelene MENESES | Artemis Racing | 41:37. | 16:39. | 3 |
| 22 | 305 | Kimberly FRANKLIN | BATC | 41:46. | 16:44. | 3 |
| 23 | 320 | Sara PEARSE | | 42:04. | 15:45. | 3 |
| 24 | 337 | Em CHIU | Pro Bike FC Women p/b Colnago | 42:08. | 16:34. | 3 |
| 25 | 341 | Rebecca IRWIN KENNEDY | Rock Creek Velo | 42:31. | 16:09. | 3 |
| 26 | 302 | Christine FELDT | Team Bikenetic | 42:53. | 15:59. | 3 |
| 27 | 314 | Sonja RODMAN | Team Bikenetic | 43:30. | 16:59. | 3 |
| 28 | 335 | Allison DREZEK | Caring for Creatures | 44:10. | 17:24. | 3 |
| 29 | 327 | Sara LAPPANO | Team Sticky Fingers | 44:13. | 16:52. | 3 |
| 30 | 347 | RACHEL GRANDORE | DAY OF REG | 44:47. | 16:57. | 3 |
| 31 | 312 | Patricia LEDESMA | Colavita-Whole Foods Market Wom | 45:14. | 17:07. | 3 |
| 32 | 330 | Dorothy MOZLOOM | Commonwealth Cycling Collective | 45:27. | 15:40. | 3 |
| 33 | 339 | Cassandra BOWEN | NCVC/UnitedHealthcare | 45:33. | 17:23. | 3 |
| 34 | 319 | Amy LUEBBERING | Team Sticky Fingers | 46:44. | 17:17. | 3 |
| 35 | 340 | Jennifer WERNER | | 47:05. | 18:23. | 3 |
| 36 | 316 | Margo WORKMAN | | 47:21. | 17:53. | 3 |
| 37 | 328 | Cameron OLIVER | NCVC/UnitedHealthcare | 48:23. | 18:25. | 3 |
| 38 | 343 | Sheila MAHADEVAN | Street Cat Cycling | 54:34. | 21:58. | 3 |
| 39 | 318 | Robyn SPANN | Team Bikenetic | 43:41. | 27:46. | 2 |
| DNF | 338 | Lauren ST. PIERRE | PBFC CX Squad | | 15:46. | 1 |
| DNF | 344 | Anna DOORENBOS | Team-Not-Team | | 19:21. | 1 |
