| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 750 | Melissa BARKER | Feedback Sports Racing | 36:00. | 9:14. | 4 | W 1-2 |
| 2 | 760 | Aubrey DRUMMOND | Spcx P/b Rk Black | 36:44. | 9:07. | 4 | W 1-2 |
| 3 | 766 | Ava HOLMGREN | Centurion Next Wave | 36:54. | 9:04. | 4 | W 1-2 |
| 4 | 753 | Melissa SEYMOUR | Team 02 Cadieux | 37:14. | 9:17. | 4 | W 1-2 |
| 5 | 800 | Lauren ZOERNER | Alpha Bicycle Compaany | 37:23. | 9:18. | 4 | W 3 |
| 6 | 816 | Danielle LARSON | Hccc | 37:40. | 9:34. | 4 | W 3 |
| 7 | 774 | Kristie AREND | Alp Racing | 38:11. | 9:39. | 4 | W 1-2 |
| 8 | 781 | Maeghan EASLER | | 38:42. | 9:40. | 4 | W 1-2 |
| 9 | 804 | Abbey OBRIEN | Harvest Racing-trek | 38:56. | 9:36. | 4 | W 3 |
| 10 | 811 | Harper BEASLEY | | 38:57. | 10:03. | 4 | W 3 |
| 11 | 757 | Robin WILLIAMS | Geoffs Bike And Ski | 39:05. | 9:28. | 4 | W 1-2 |
| 12 | 771 | Venny ALUB | Cantu Cycling Wheels | 39:24. | 9:42. | 4 | W 1-2 |
| 13 | 762 | Emily NORDAHL | Team Wheel & Sprocket | 39:27. | 10:00. | 4 | W 1-2 |
| 14 | 777 | Karen BALIKA | Alpha Bicycle Co./groove Suburu | 39:31. | 10:01. | 4 | W 1-2 |
| 15 | 802 | Kathryn MCDICKEN | Asu Junior Development | 39:37. | 9:52. | 4 | W 3 |
| 16 | 551 | Linda COOPER | Sakari Sushi Lounge Race Team P | 39:57. | 9:42. | 4 | W 45+ |
| 17 | 785 | Jen RAGAN-MARLOWE | Matrix/rbm | 39:59. | 10:07. | 4 | W 1-2 |
| 18 | 813 | Sarah WILLIAMS | Vanderkitten | 40:02. | 10:06. | 4 | W 3 |
| 19 | 801 | Valerie HYRMAN | Hagerty Cycling Team | 40:16. | 10:01. | 4 | W 3 |
| 20 | 821 | Julia ENGELSTED | | 40:36. | 10:23. | 4 | W 3 |
| 21 | 772 | Carly THOMSEN | Orion Racing/orion Racing P/b B | 40:45. | 10:28. | 4 | W 1-2 |
| 22 | 805 | Reagan LONG | Boulder Junior Cycling | 41:09. | 10:28. | 4 | W 3 |
| 23 | 784 | Kathryn THOMPSON | | 41:23. | 10:24. | 4 | W 1-2 |
| 24 | 864 | Ashley MILLER | Goldenrod Pastries | 41:47. | 10:27. | 4 | W 4-5 |
| 25 | 773 | Leah LOGGIN | Tsf | 41:47. | 10:52. | 4 | W 1-2 |
| 26 | 814 | Elizabeth FRANCZYK | Palomino American Cyclocross | 42:29. | 10:45. | 4 | W 3 |
| 27 | 808 | Darian NAGLE-GAMM | University Of Iowa Hospitals He | 42:43. | 10:42. | 4 | W 3 |
| 28 | 553 | Jessica WHITON | Xxx Racing-athletico | 42:58. | 11:05. | 4 | W 45+ |
| 29 | 819 | Jessica WITTWER | Gopher Wheelmen/county Cycles | 43:07. | 10:46. | 4 | W 3 |
| 30 | 862 | Michelle HENRY | Palmares Racing | 44:25. | 10:46. | 4 | W 4-5 |
| 31 | 788 | Alyson GREEN | Womens Freestate Racing | 44:39. | 11:25. | 4 | W 1-2 |
| 32 | 878 | Kristi PROUGH | Soundpony Triad Bank | 44:49. | 11:00. | 4 | W 4-5 |
| 33 | 561 | Julie THOMPSON | Mspeedwax.com | 45:12. | 11:14. | 4 | W 45+ |
| 34 | 552 | Sarah BARBER | Crandic Racing Team | 45:32. | 11:08. | 4 | W 45+ |
| 35 | 812 | Sarah LARSON | Lady Gnar Shredders | 45:37. | 10:59. | 4 | W 3 |
| 36 | 870 | Olivia CROSKEY | Gravel Scouts | 45:53. | 11:21. | 4 | W 4-5 |
| 37 | 822 | Emily HOUTCHENS | Orion Racing P/b Borah Teamwear | 35:06. | 10:52. | 3 | W 3 |
| 38 | 866 | Lucia SMITH | Brazen Dropouts | 35:37. | 11:45. | 3 | W 4-5 |
| 39 | 867 | Crista GILMORE | Palmares | 36:19. | 12:30. | 3 | W 4-5 |
| 40 | 556 | Kathleen PORTER | Twisted Spokes | 37:05. | 12:26. | 3 | W 45+ |
| 41 | 818 | Ingrid ANDERSON | Iowa City Cycling Club | 38:24. | 13:14. | 3 | W 3 |
| 42 | 555 | Tara COADY | Crandic Racing | 38:28. | 12:07. | 3 | W 45+ |
| 43 | 872 | Marylou GERALTS | Vc Bike Sport | 38:57. | 12:40. | 3 | W 4-5 |
| 44 | 559 | Lara FISHER | Kuhl Cycling Team | 40:01. | 13:41. | 3 | W 45+ |
| 45 | 558 | Rebecca BASTIAENS | | 44:25. | 14:41. | 3 | W 45+ |
| 46 | 875 | Kelly LAMBETH | Palamino American Cyclocross | 49:32. | 15:51. | 3 | W 4-5 |
| 47 | 560 | Kirschen SEAH | Crandic Racing Team | 49:35. | 16:17. | 3 | W 45+ |
| DNF | 759 | Amanda SCHAAP | Speed Merchants P/b Wynalda Pac | | 10:11. | 1 | W 1-2 |
| DNF | 879 | Toni CABEZAS | Elektra Racing | | 15:09. | 2 | W 4-5 |
