| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 544 | Jennifer METTLER | NorthStar Development Cycling | 45:22. | 8:52. | 5 | W 3 |
| 2 | 532 | Maeghan EASLER | Ames Velo | 46:12. | 9:23. | 5 | W 3 |
| 3 | 548 | Danielle LARSON | Holiday Camp Cycling Club | 46:25. | 9:14. | 5 | W 3 |
| 4 | 508 | Linda COOPER | Sakari Sushi Lounge Race Team p | 47:44. | 9:43. | 5 | W 3 |
| 5 | 503 | Abbey OBRIEN | Harvest Racing | 48:58. | 9:31. | 5 | W 3 |
| 6 | 505 | Valerie HYRMAN | Hagerty Cycling Team | 39:43. | 9:46. | 4 | W 3 |
| 7 | 547 | Cynthia BANNINK | | 40:18. | 9:37. | 4 | W 3 |
| 8 | 502 | Kathryn MCDICKEN | ASU Junior Development | 40:18. | 9:08. | 4 | W 3 |
| 9 | 524 | Victoria CANE | Team Clark Logic | 40:44. | 10:00. | 4 | W 3 |
| 10 | 509 | Haley ENGLE | Half Acre Cycling | 40:59. | 9:55. | 4 | W 3 |
| 11 | 517 | Meghan MASON | Power Fix CX | 41:26. | 10:11. | 4 | W 3 |
| 12 | 513 | Anna LUCKOW | Loon State Cyclists | 41:31. | 10:02. | 4 | W 3 |
| 13 | 539 | Amy SCHULTZ | L5 Racing | 41:59. | 10:14. | 4 | W 3 |
| 14 | 507 | Jennifer GROEN | Half Acre Cycling | 42:10. | 10:23. | 4 | W 3 |
| 15 | 526 | Lily ENSIGN | NorthStar Development Cycling | 42:21. | 10:27. | 4 | W 3 |
| 16 | 542 | Sarah WILLIAMS | Vanderkitten | 42:34. | 10:32. | 4 | W 3 |
| 17 | 522 | Eleanor THERMANSEN | Loon State Cyclists | 43:01. | 10:15. | 4 | W 3 |
| 18 | 515 | Harper BEASLEY | Trek Midwest Team | 43:11. | 10:37. | 4 | W 3 |
| 19 | 521 | Morgan STYER | Foxy Moxy | 44:27. | 10:37. | 4 | W 3 |
| 20 | 527 | Erin AYALA | Loon State Cyclists | 44:51. | 10:56. | 4 | W 3 |
| 21 | 545 | Ashley MOORE | Spidermonkey Cycling | 45:09. | 11:24. | 4 | W 3 |
| 22 | 538 | Tori RIEMERSMA | Landis/Trek | 45:20. | 11:17. | 4 | W 3 |
| 23 | 525 | Jennifer MOSLEY | Half Acre Cycling | 45:55. | 11:40. | 4 | W 3 |
| 24 | 511 | Elizabeth FRANCZYK | | 46:04. | 11:25. | 4 | W 3 |
| 25 | 543 | Lilly FERGUSON | Central Junior Cycling Devo | 47:36. | 12:36. | 4 | W 3 |
| 26 | 510 | April DOMBROWSKI | Team Wheel & Sprocket | 47:47. | 11:40. | 4 | W 3 |
| 27 | 519 | Robin SUND | Zealous Racing | 48:50. | 11:55. | 4 | W 3 |
| 28 | 530 | Brooklyn RADDIN | LOGIK | Junior Development Team | 39:18. | 12:57. | 3 | W 3 |
| 29 | 516 | Kathryn GOFFIN | Iowa City Cycling Club | 45:44. | 23:40. | 2 | W 3 |
| DNF | 529 | Ingrid ANDERSON | University of Iowa Heart and Va | | 13:03. | 2 | W 3 |
