| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 13 | Abby STRIGEL | | 45:01. | 9:22. | 5 | W 1-2 |
| 2 | 2 | Sydney GUAGLIARDO | | 45:17. | 9:22. | 5 | W 1-2 |
| 3 | 1 | Katherine ISERMANN | PSIMET Racing | 46:39. | 9:34. | 5 | W 1-2 |
| 4 | 10 | Andrea CYR | Velocause Centraal Cycling | 47:13. | 9:56. | 5 | W 1-2 |
| 5 | 9 | Morgan STERN | Comrade Cycles Racing | 48:13. | 9:39. | 5 | W 1-2 |
| 6 | 7 | Lauren WISCOMB | | 48:14. | 9:40. | 5 | W 1-2 |
| 7 | 4 | Ashley HEIDENREICH | Half Acre Cycling | 48:31. | 10:05. | 5 | W 1-2 |
| 8 | 8 | Antonia LEAL | | 48:38. | 9:48. | 5 | W 1-2 |
| 9 | 64 | Lauren HARKNESS | Velocause Centraal Cycling | 48:51. | 9:32. | 5 | W 3-4 |
| 10 | 14 | Lauren WISSMAN | | 49:29. | 10:11. | 5 | W 1-2 |
| 11 | 11 | Lindsay KNIGHT | Tenspeed Hero | 49:47. | 10:02. | 5 | W 1-2 |
| 12 | 44 | Rachel WILLS | | 50:27. | 10:04. | 5 | W 3-4 |
| 13 | 56 | Meesa MAENG | Union Cycling Team | 50:36. | 10:14. | 5 | W 3-4 |
| 14 | 5 | Courtney TANNER | | 51:00. | 12:43. | 5 | W 1-2 |
| 15 | 72 | Beth CHRISTIANSEN | Union Cycling Team | 51:29. | 10:37. | 5 | W 3-4 |
| 16 | 12 | Christine THORNBURG | Velosmith Bicycle Studio | 41:05. | 10:40. | 4 | W 1-2 |
| 17 | 48 | Kimberly STOVER | The Pony Shop | 41:32. | 10:23. | 4 | W 3-4 |
| 18 | 68 | Haley ENGLE | Half Acre Cycling | 41:37. | 10:36. | 4 | W 3-4 |
| 19 | 3 | Amanda SCHAAP | Speed Merchants | 42:27. | 14:50. | 4 | W 1-2 |
| 20 | 45 | Kate VERMANN | Billy Goat Racing Team | 42:45. | 10:18. | 4 | W 3-4 |
| 21 | 73 | Olivia BEATY | | 42:56. | 10:58. | 4 | W 3-4 |
| 22 | 54 | Ashley MOORE | Bloomington Cycle Racing Team | 43:10. | 10:20. | 4 | W 3-4 |
| 23 | 67 | Charlotte ERICKSON | Dirty Squirrels Racing | 43:17. | 10:53. | 4 | W 3-4 |
| 24 | 61 | Lauren JACOBS | xXx Racing | 43:41. | 11:02. | 4 | W 3-4 |
| 25 | 50 | Sharon SHACHAR | | 43:42. | 10:52. | 4 | W 3-4 |
| 26 | 53 | Jennifer MOSLEY | Half Acre Cycling | 43:47. | 10:53. | 4 | W 3-4 |
| 27 | 52 | Caroline RODRIGUEZ-NELSON | INTENT | 43:51. | 10:53. | 4 | W 3-4 |
| 28 | 6 | Kristen HOSEY | UPB Cycling Club | 44:20. | 15:07. | 4 | W 1-2 |
| 29 | 55 | Lorraine MCCARTHY | Force Power Racing | 44:38. | 10:50. | 4 | W 3-4 |
| 30 | 66 | Elizabeth FRANCZYK | | 44:55. | 11:32. | 4 | W 3-4 |
| 31 | 49 | Laura SHIELDS | BFF Bikes | 45:09. | 11:30. | 4 | W 3-4 |
| 32 | 51 | Carrie SCHOENEBERGER | Main Street Bicycles Sasquatch | 45:24. | 11:39. | 4 | W 3-4 |
| 33 | 60 | Gina JOHNSON | | 45:47. | 11:33. | 4 | W 3-4 |
| 34 | 71 | Lauren CONROY | Comrade Cycles Racing | 46:29. | 11:40. | 4 | W 3-4 |
| 35 | 69 | Kathleen DEAMICO | Union Cycling Team | 47:36. | 11:59. | 4 | W 3-4 |
| 36 | 58 | Ramona KUNA | ABD Cycling Club | 49:20. | 12:50. | 4 | W 3-4 |
| 37 | 70 | Katerina CRIVELLO | INTENT | 49:49. | 12:55. | 4 | W 3-4 |
| 38 | 57 | Trisha KURTZ | Spidermonkey Cycling | 50:00. | 12:42. | 4 | W 3-4 |
| 39 | 46 | Michele UNDERWOOD | Mox Multisport | 51:22. | 13:28. | 4 | W 3-4 |
| 40 | 43 | Isabel REYES-TODD | | 53:14. | 13:49. | 4 | W 3-4 |
| 41 | 63 | Emily HEAVEY | Half Acre Cycling | 54:07. | 14:02. | 4 | W 3-4 |
| 42 | 42 | Ali RAIZIN | | 41:48. | 15:27. | 3 | W 3-4 |
| DNF | 75 | Laura ALAGNA | Union Cycling Team | | 11:35. | 1 | W 3-4 |
| DNF | 62 | Erica JACOBO | Team Wisconsin | | 12:27. | 3 | W 3-4 |
| DNF | 59 | Denise KARLIN | Spidermonkey Cycling | | 13:57. | 3 | W 3-4 |
