| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 797 | Maddie WOOLLEY | | 37:23. | 37:23 |
| 2 | 801 | Marissa CARTER | Team Mack Racing | 37:23. | s.t. |
| 3 | 795 | Finn TAYLOR | City Title Cycling Team | 37:23. | s.t. |
| 4 | 763 | Noni BERTAGNOLI | Dallas Racing | 37:23. | s.t. |
| 5 | 817 | Maeghan EASLER | | 37:23. | s.t. |
| 6 | 799 | Emily GASS | Urban South Racing | 37:24. | s.t. |
| 7 | 766 | Jessica BROOKS | Pandemonium Cycling p/b Hodges | 37:24. | s.t. |
| 8 | 789 | Sarah SCHUETTER | | 37:24. | s.t. |
| 9 | 791 | Rachel SMITH | Crush Racing | 37:24. | s.t. |
| 10 | 760 | Anna AFFIAS | | 37:24. | s.t. |
| 11 | 783 | Erin OBRADOVICH | | 37:24. | s.t. |
| 12 | 787 | Christine ROETTGER | | 37:24. | s.t. |
| 13 | 805 | Claire REINBOLD | | 37:24. | s.t. |
| 14 | 790 | Kate SEILER | Sho-Air TWENTY20 Development | 37:24. | s.t. |
| 15 | 774 | Jessica HE | | 37:24. | s.t. |
| 16 | 794 | Francesca STUBBINS | Pastaria - Big Shark Racing Tea | 37:24. | s.t. |
| 17 | 761 | Katie AREHART-ROSE | Naked Women37:25. | s.t. | 37:25. |
| 18 | 788 | Leigh RUSTON | Marx-Bensdorf / BPC Cycling Tea | 37:25. | s.t. |
| 19 | 796 | Katie VAN HOOK | Pastaria - Big Shark Racing Tea | 37:25. | s.t. |
| 20 | 810 | Lily ENSIGN | NorthStar Development Cycling | 37:25. | s.t. |
| 21 | 807 | Lauren KOON | Team Cadence Cyclery p/b Audi M | 37:25. | s.t. |
| 22 | 798 | Melissa GOMES | Quantum Solutions Racing | 37:25. | s.t. |
| 23 | 780 | Marianne MELLING | GP Velotek | 37:25. | s.t. |
| 24 | 778 | Lisa LIM | BFF Bikes | 37:26. | s.t. |
| 25 | 792 | Faith SOLLER | | 37:26. | s.t. |
| 26 | 765 | Cynthia BRACKER | Womens Free State Racing | 37:26. | s.t. |
| 27 | 764 | Tanya BOULANOVA | ButcherBox Cycling | 37:26. | s.t. |
| 28 | 803 | Sarah HOOTS | Sorella Cycling p/b Hincapie Sp | 37:26. | s.t. |
| 29 | 770 | Amy DAVISON | | 37:27. | s.t. |
| 30 | 811 | Ashley HEIDENREICH | Half Acre Cycling | 37:27. | s.t. |
| 31 | 782 | C??Line OBERHOLZER | | 37:27. | s.t. |
| 32 | 781 | Ashley MILLER | GP Velotek | 37:27. | s.t. |
| 33 | 767 | Kelsey CAMPBELL DOLLAGHAN | BFF Bikes | 37:27. | s.t. |
| 34 | 815 | Veronika REDENSEK | | 37:28. | s.t. |
| 35 | 772 | Kate GUSKE | GP Velotek | 37:28. | s.t. |
| 36 | 776 | Lori HINDS | | 37:28. | s.t. |
| 37 | 793 | Patience STOLLER | Nashville Local Cycling | 37:28. | s.t. |
| 38 | 779 | Sara LITTLEMASTERSON | #iridestigmafree | 37:29. | s.t. |
| 39 | 771 | Aylannah DYLAG | GP Velotek | 37:29. | s.t. |
| 40 | 816 | Lauren POINTER | Big Tree Cycling | 37:31. | 8 |
| 41 | 768 | Angela CAREY | Naked Womens Racing | 37:35. | 12 |
| 42 | 814 | Jade HODGE | | 37:35. | s.t. |
| 43 | 777 | Ashley KING | Palmares Racing | 37:36. | s.t. |
| 44 | 800 | Renee DYKSTRA | Lindenwood University | 37:57. | 34 |
| 45 | 773 | Katie HARRER | Palmares Racing | 38:10. | 47 |
| 46 | 812 | Janette BARBER | The Cyclery Racing Team | 38:15. | 52 |
| @1Lap | 785 | Jennifer PIKO | KCBC Racing | | |
| @1Lap | 784 | Heather PATTON | Korte Hammer Down Racing | | |
| @2Lap | 804 | Vanessa BUCCELLA | ALP Cycles Racing | | |
| @2Lap | 809 | Fiona RICHARDS | | | |
| DNF | 775 | Lisa HILLEREN | BFF Bikes | | |
