| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 202 | Megan JASTRAB | iCademy | 1:07:44. | 1:07:44 |
| 2 | 219 | Faith MONTREUIL | Gray Goat - Bullseye Total Medi | 1:07:44. | s.t. |
| 3 | 220 | Carmen DAVIDSON | Andrie Junior Development Team | 1:07:44. | s.t. |
| 4 | 229 | Zoe TA-PEREZ | Sho-Air TWENTY20 Development | 1:07:44. | s.t. |
| 5 | 206 | Olivia CUMMINS | Melton Design Build/Cari Higgin | 1:07:45. | s.t. |
| 6 | 223 | Bridget TOOLEY | Latinis Bicycle Repair | 1:07:45. | s.t. |
| 7 | 217 | Makayla MACPHERSON | Sho-Air TWENTY20 Development | 1:07:45. | s.t. |
| 8 | 222 | Elizabeth HARDEN | Papa Johns Racing p/b TREK | 1:07:45. | s.t. |
| 9 | 230 | Kaia SCHMID | New England Devo p/b Cadence We | 1:07:45. | s.t. |
| 10 | 218 | Jennifer METTLER | NorthStar Development Cycling | 1:07:46. | s.t. |
| 11 | 221 | Ava SYKES | LUX/Sideshow p/b Specialized | 1:11:33. | 3:49 |
| 12 | 231 | Lauren ZOERNER | Alpha Bicycle Co.- Groove Subar | 1:11:33. | s.t. |
| 13 | 227 | Kate SEILER | Sho-Air TWENTY20 Development | 1:11:33. | s.t. |
| 14 | 210 | Lily ENSIGN | NorthStar Development Cycling | 1:11:34. | s.t. |
| 15 | 215 | Kayla HANKINS | Velo Track Cycling | 1:11:34. | s.t. |
| 16 | 224 | Cassie BOPF | Rock Creek Velo | 1:11:38. | 3:54 |
| 17 | 207 | Gabriella DIXON | Mr. Greens p/b Top Step Develop | 1:14:45. | 7:01 |
| 18 | 204 | Allison MCCURRY | Team Louisville Junior Cycling | 1:14:45. | s.t. |
| 19 | 225 | Taylor JOHNSON | GS Forza Cycling | 1:15:09. | 7:25 |
| 20 | 212 | Kloe PFANNMULLER | BYRDS (Boise Young Rider Dev Sq | 1:16:32. | 8:48 |
| 21 | 213 | Allyson SELANDER | BYRDS (Boise Young Rider Dev Sq | 1:16:34. | 8:50 |
| 22 | 214 | Rosalyn WILLEY | Team Louisville Junior Cycling | 1:19:32. | 11:48 |
| 23 | 216 | Megan LESTER | Diablo Cycling | 1:19:33. | s.t. |
| 24 | 209 | Danielle SHUMSKAS | BIG Picture Cycling | 1:19:33. | s.t. |
| 25 | 211 | Cara KING | Young Medalists | 1:20:48. | 13:04 |
| 26 | 201 | Kensa KOEPPEN | Team Wheel & Sprocket | 1:21:35. | 13:51 |
| 27 | 226 | Renee MILLER | All American Bicycle Center Cyc | 1:26:25. | 18:41 |
| 28 | 208 | Mariana RINALDO | Bike Loft East | 1:26:29. | 18:45 |
