| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 223 | Abigail YOUNGWERTH | TWENTY20 development | 28:05. | 28:05 |
| 2 | 208 | Karissa LAMB | PLAN7 DS Cycling Team | 28:15. | 10 |
| 3 | 213 | Sarah MUENCH | SkyFlash Racing | 28:16. | s.t. |
| 4 | 212 | Shanin MILLER | Hangar 15 Bicycles | 28:16. | s.t. |
| 5 | 221 | Kelsey SPIEGEL | Team Digestive Health Clinic Bo | 28:17. | s.t. |
| 6 | 216 | Kloe PFANNMULLER | BYRDS | 28:18. | s.t. |
| 7 | 227 | Kate JUNGNICKEL | SJBC | 28:18. | s.t. |
| 8 | 211 | Liesl MILAN | Team 10 Barrel | 28:18. | s.t. |
| 9 | 220 | Allyson SELANDER | BYRDS | 28:19. | s.t. |
| 10 | 207 | Catie JUNGMANN | | 28:19. | s.t. |
| 11 | 204 | Molly ELLIOTT | BYRDS | 28:19. | s.t. |
| 12 | 203 | Christine EDWARDS | Team Bobs-Bicycles.com | 28:19. | s.t. |
| 13 | 200 | Tiedji BAKER | Team Digestive Health Clinic Bo | 28:19. | s.t. |
| 14 | 214 | Jordyn OLDENBURG | BYRDS | 28:19. | s.t. |
| 15 | 222 | Ami STUART | | 28:20. | s.t. |
| 16 | 226 | Kelli SAMUELSON | | 28:20. | s.t. |
| 17 | 215 | Mahika PETERSON | BYRDS | 28:20. | s.t. |
| 18 | 218 | Heather RAMSDELL | Riders Roost | 28:21. | s.t. |
| 19 | 210 | Jannalyn LUTTRELL | | 28:40. | 35 |
| 20 | 202 | Nadine CARTER | Team Digestive Health Clinic Bo | 29:10. | 1:05 |
| @1Lap | 201 | Kathryn BERTANY | Big Picture Cycling | | |
| @1Lap | 224 | Caroline DIVIETRO | BYRDS | | |
| @1Lap | 217 | Nekane POWELL | BYRDs | | |
| @1Lap | 209 | Sage LEMAY | BYRDS | | |
| @3Lap | 219 | An SECRIST | BYRDS | | |
