| Rank | Bib | Name | Affiliation | Time | Gap | Category |
| 15 | Megan JASTRAB | iCademy | 55:53. | 55:53 | W PRO |
| 33 | Valentina SCANDOLARA | Roxsolt Attaquer | 55:54. | 1 | W PRO |
| 45 | Coryn RIVERA | UCI WPT: Hagens Berman | Superm | 55:54. | 1 | W PRO |
| 40 | Erika VARELA | CONADE-Visit Mexico-Specialized | 55:54. | 1 | W PRO |
| 38 | Starla TEDDERGREEN | UCI WPT: Hagens Berman | Superm | 55:54. | 1 | W PRO |
| 27 | Edwige PITEL | GMC38 | 55:54. | 1 | W PRO |
| 29 | Shelby REYNOLDS | Monster Media Elite Women | 55:54. | 1 | W PRO |
| 5 | Renata BUCHER | Roxsolt Attaquer | 55:55. | 2 | W PRO |
| 8 | Angelica FRAYRE | SDBC/EMERALD TEXTILES p/b UC Cy | 55:55. | 2 | W PRO |
| 18 | Jennifer LUEBKE | UCI WPT: Hagens Berman | Superm | 55:55. | 2 | W PRO |
| 22 | Esther MEISELS | | 55:55. | 2 | W PRO |
| 21 | Kasey MCEVOY | Landis/Trek | 55:55. | 2 | W PRO |
| 39 | Lizbeth URENO ARMAS | Lagrange | 55:55. | 2 | W PRO |
| 34 | Pamela SCHUSTER | Skyflash Racing | 55:56. | 3 | W PRO |
| 23 | Peta MULLENS | Hagens Berman Supermint | 55:56. | 3 | W PRO |
| 9 | Daniela GARCIA | Velo Club La Grange | 55:56. | 3 | W PRO |
| 1 | Julyn AGUILA | CONADE-Visit Mexico-Specialized | 55:56. | 3 | W PRO |
| 41 | Ellie WOOD | Landis/Trek | 55:57. | 4 | W PRO |
| 7 | Alexi COSTA | Heatwave CC | 55:57. | 4 | W PRO |
| 3 | Jessica BAUMGARDNER | Stinner Bicycle Club Elite Raci | 55:57. | 4 | W PRO |
| 2 | Justine BARROW | Roxsolt Attaquer | 55:57. | 4 | W PRO |
| 30 | Jenny RIOS | SDBC/EMERALD TEXTILES p/b UC Cy | 55:57. | 4 | W PRO |
| 44 | Kate WILSON | Ironfly | 55:58. | 5 | W PRO |
| 4 | Ginger BOYD | Stinner Bicycle Club Elite Raci | 55:58. | 5 | W PRO |
| 43 | Mia CHEESEMAN | Team Wheel & Sprocket | 55:58. | 5 | W PRO |
| 36 | Philippa SUTTON | None | 55:59. | 6 | W PRO |
| 12 | Ariadna GUTIERREZ ARZALUZ | CONADE- Specialized | 55:59. | 6 | W PRO |
| 20 | Makayla MACPHERSON | Sho-Air TWENTY20 Development | 55:59. | 6 | W PRO |
| 25 | Lori NEDESCU | | 56:00. | 7 | W PRO |
| 37 | Zoe TA-PEREZ | Sho-Air TWENTY20 Development | 56:00. | 7 | W PRO |
| 24 | Katherine NADLER | SDBC/EMERALD TEXTILES p/b UC Cy | 56:00. | 7 | W PRO |
| 6 | Tarah COLE | UCI WPT: Hagens Berman | Superm | 56:01. | 8 | W PRO |
| 11 | Lindsay GOLDMAN | UCI WPT: Hagens Berman | Superm | 56:01. | 8 | W PRO |
| 14 | Trina JACOBSON | SDBC/EMERALD TEXTILES p/b UC Cy | 56:01. | 8 | W PRO |
| 16 | Aliesha LARSEN | DNA Cycling Team | 56:01. | 8 | W PRO |
| 17 | Veronica LEAL | CONADE-Visit M?xico-Specialized | 56:02. | 9 | W PRO |
| 13 | Ana HERNANDEZ | CONADE-Visit M?xico-Specialized | 56:05. | 12 | W PRO |
| 10 | Emily GEORGESON | Monster Media Elite Women | 56:06. | 13 | W PRO |
| 28 | Liza RACHETTO | UCI WPT: Hagens Berman | Superm | 56:17. | 24 | W PRO |
| 42 | Heather ODOM | SDBC/EMERALD TEXTILES p/b UC Cy | | | W PRO |
| 31 | Betsabee SALOMON | Stone House Group p/b Monster M | | | W PRO |
| 26 | Irena OSSOLA | New Mexico Bicycle Racing Assoc | | | W PRO |
