| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 230 | Jennifer BINKOWSKI | | 43:01. | 8:19. | 5 | W 4 |
| 2 | 206 | Angie ARMSTEAD | Rasmussen Bike Shop | 37:29. | 8:46. | 4 | W 4 |
| 3 | 201 | samantha SCIPIO | Comrade Cycles Racing | 37:34. | 8:47. | 4 | W 4 |
| 4 | 200 | Michelle MOORE | | 37:45. | 9:03. | 4 | W 4 |
| 5 | 202 | Alisa TOBIAS | | 37:59. | 8:55. | 4 | W 4 |
| 6 | 205 | Alexa STIERWALT | Lionhearts Junior Racing | 38:04. | 9:14. | 4 | W 4 |
| 7 | 238 | Sarah MAKOSKI | Brazen Dropouts Cycling Team | 38:06. | 9:17. | 4 | W 4 |
| 8 | 299 | Michaela ALBANESE | Team Laser Cats | 38:28. | 9:08. | 4 | W 4 |
| 9 | 213 | Olivia COPE | Brazen Dropouts Cycling Team | 38:41. | 9:40. | 4 | W 4 |
| 10 | 204 | Kristina CAUDLE | BFF Bikes | 38:41. | 9:54. | 4 | W 4 |
| 11 | 203 | Tania GREGORY | Colonial Bicycle Company | 38:59. | 9:12. | 4 | W 4 |
| 12 | 236 | Liz HORNING | | 39:12. | 9:09. | 4 | W 4 |
| 13 | 207 | Amy ANCHETA | Spidermonkey Cycling | 39:22. | 9:39. | 4 | W 4 |
| 14 | 212 | Robin SUND | Zealous Racing | 39:25. | 9:54. | 4 | W 4 |
| 15 | 211 | Genna BROCK | Team Louisville | 40:11. | 10:14. | 4 | W 4 |
| 16 | 218 | Heather GREGOIRE | Angry Monkeys Racing | 40:17. | 10:12. | 4 | W 4 |
| 17 | 297 | Trina STALOCH | Freewheel Bike | 40:29. | 9:36. | 4 | W 4 |
| 18 | 219 | Elizabeth FRANCZYK | Palomino American Cyclocross | 40:34. | 9:51. | 4 | W 4 |
| 19 | 223 | Kelsey WEBB | | 40:48. | 9:30. | 4 | W 4 |
| 20 | 217 | Keri TURNER | Loon State Cyclists | 40:57. | 9:33. | 4 | W 4 |
| 21 | 234 | Ashley MILLER | Goldenrod Pastries CX | 41:17. | 9:44. | 4 | W 4 |
| 22 | 294 | Anna AFFIAS | | 41:50. | 10:37. | 4 | W 4 |
| 23 | 210 | Kathryn GOFFIN | Iowa City Cycling Club | 42:04. | 10:09. | 4 | W 4 |
| 24 | 221 | Jill SUURMEYER | Hub Cycling Team | 42:24. | 10:15. | 4 | W 4 |
| 25 | 214 | Melissa ZAMMIT | | 42:55. | 10:43. | 4 | W 4 |
| 26 | 293 | Kathleen DEAMICO | Union Cycling Team | 43:02. | 10:25. | 4 | W 4 |
| 27 | 240 | Mikayla RHONE | | 43:21. | 10:56. | 4 | W 4 |
| 28 | 208 | Charlie GROSSENHYDER | | 43:22. | 10:59. | 4 | W 4 |
| 29 | 239 | Anna PICKEREL | | 43:57. | 11:06. | 4 | W 4 |
| 30 | 220 | Ellen FOSTER | | 44:11. | 10:56. | 4 | W 4 |
| 31 | 237 | Jenny KREPELA | | 34:59. | 11:30. | 3 | W 4 |
| 32 | 209 | Kelly LAMBETH | Bens Cycle/Milwaukee Bicycle C | 35:57. | 11:51. | 3 | W 4 |
| 33 | 232 | Courtney NORMAN | Freewheel Bike | 36:44. | 13:01. | 3 | W 4 |
| 34 | 225 | Rebecca BASTIAENS | | 37:16. | 12:26. | 3 | W 4 |
| 35 | 229 | Linda LASTAYO | KUHL Cycling Team | 37:24. | 12:26. | 3 | W 4 |
| 36 | 224 | Lara FISHER | KUHL Cycling Team | 37:51. | 13:02. | 3 | W 4 |
| 37 | 233 | Morgan FISHER | Gryphon Velo Racing | 38:16. | 13:04. | 3 | W 4 |
| 38 | 216 | Kirschen SEAH | New Pioneer Food Co-op | 41:13. | 13:36. | 3 | W 4 |
| DNF | 235 | Susan BERNATAS | | | 9:09. | 3 | W 4 |
| DNF | 296 | Willow OLSEN | Central Junior Cycling Devo | | 11:37. | 3 | W 4 |
| DNF | 222 | Kimberly SCHEER | UPB Cycling Club | | 10:58. | 2 | W 4 |
