| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 2 | Rachel RUBINO | | 38:22. | 7:50. | 5 | W 1-2 |
| 2 | 6 | Megan BARR | Angry Catfish | 39:21. | 8:36. | 5 | W 1-2 |
| 3 | 9 | Lily WILLIAMS | | 39:30. | 7:57. | 5 | W 1-2 |
| 4 | 3 | Regina LEGGE | Trek Cyclocross Collective | 39:42. | 8:04. | 5 | W 1-2 |
| 5 | 45 | Abby STRIGEL | | 39:42. | 7:55. | 5 | W 1-2 |
| 6 | 11 | Erin FELDHAUSEN | Trek Midwest Team | 39:43. | 7:55. | 5 | W 1-2 |
| 7 | 18 | Raylyn NUSS | Maplewood Bicycle St. Louis | 39:54. | 8:06. | 5 | W 1-2 |
| 8 | 23 | Carol SEIPP | | 40:02. | 8:06. | 5 | W 1-2 |
| 9 | 99 | Alexandra CAMPBELLFORTE | Vanderkitten CX | 40:09. | 8:08. | 5 | W 1-2 |
| 10 | 8 | Sydney GUAGLIARDO | | 40:13. | 7:56. | 5 | W 1-2 |
| 11 | 97 | Katie ISERMANN | PSIMET Racing | 40:17. | 8:07. | 5 | W 1-2 |
| 12 | 48 | Fiona MORRIS | | 40:19. | 8:09. | 5 | W 1-2 |
| 13 | 35 | Julie SAELER | Trek Cyclocross Collective | 40:25. | 8:10. | 5 | W 1-2 |
| 14 | 7 | Catherine MOORE | Trek Cyclocross Collective | 40:37. | 8:23. | 5 | W 1-2 |
| 15 | 13 | Amanda SCHAAP | Speed Merchants | 40:37. | 8:19. | 5 | W 1-2 |
| 16 | 39 | Carly THOMSEN | Midwest Cycling Trek Stores Wom | 41:02. | 8:18. | 5 | W 1-2 |
| 17 | 30 | Kelli RICHTER | PSIMET Racing | 41:19. | 8:18. | 5 | W 1-2 |
| 18 | 96 | Molly CLARK-OIEN | Queen City Cycling/Sanford Heal | 41:19. | 8:14. | 5 | W 1-2 |
| 19 | 17 | Anya MALARSKI | Transitions LifeCare p/b Garnea | 41:22. | 8:37. | 5 | W 1-2 |
| 20 | 100 | Faith MONTREUIL | Team Louisville | 41:29. | 8:14. | 5 | W 3 |
| 21 | 26 | Meredith PETERSON | Neff Cycle Service | 41:45. | 8:21. | 5 | W 1-2 |
| 22 | 27 | Sarah SZEFI | PSIMET Racing | 41:48. | 8:21. | 5 | W 1-2 |
| 23 | 12 | Emily MOLDEN | Nantucket Velo | 41:56. | 8:31. | 5 | W 1-2 |
| 24 | 101 | Melissa SEYMOUR | Team O2 | 42:06. | 8:21. | 5 | W 3 |
| 25 | 15 | April BEARD | Trek Midwest Team | 42:10. | 8:16. | 5 | W 1-2 |
| 26 | 109 | Anne MAYER | Trek Midwest Team | 42:12. | 8:14. | 5 | W 3 |
| 27 | 38 | Rebecca MIKRUT | Skunkworks Racing | 42:13. | 8:28. | 5 | W 1-2 |
| 28 | 28 | Courtney TANNER | Union Cycling Team | 42:19. | 8:36. | 5 | W 1-2 |
| 29 | 22 | Julie PHELPS | Gryphon Velo Racing | 42:24. | 8:22. | 5 | W 1-2 |
| 30 | 25 | Courtney ONEILL | xXx Racing | 42:25. | 8:41. | 5 | W 1-2 |
| 31 | 29 | Jodie PRESTINE | Bens Cycle/Milwaukee Bicycle C | 42:27. | 8:31. | 5 | W 1-2 |
| 32 | 33 | Jennifer YOUNGWORTH | | 42:38. | 8:39. | 5 | W 1-2 |
| 33 | 104 | Lily PECK | Womens CX Project | 42:45. | 8:42. | 5 | W 3 |
| 34 | 230 | Jennifer BINKOWSKI | | 43:01. | 8:19. | 5 | W 4 |
| 35 | 24 | Elisabeth REINKORDT | Team Laser Cats | 43:05. | 8:31. | 5 | W 1-2 |
| 36 | 41 | Lorena BROWN | Wells Fargo Advisors | 43:08. | 8:43. | 5 | W 1-2 |
| 37 | 115 | Abbey OBRIEN | Midwest Cycling Trek Stores Wom | 43:12. | 8:35. | 5 | W 3 |
| 38 | 32 | Annicka CAMPBELL-DOLLAGHAN | BFF Bikes | 43:13. | 9:00. | 5 | W 1-2 |
| 39 | 106 | Linda COOPER | Sakari Race Team | 43:28. | 8:56. | 5 | W 3 |
| 40 | 110 | Lauren WISCOMB | | 43:40. | 8:36. | 5 | W 3 |
| 41 | 42 | Jamie ERTL | KS Energy Services / MOSH / Tea | 43:53. | 9:21. | 5 | W 1-2 |
| 42 | 34 | Jessica WHITTON | | 43:57. | 8:41. | 5 | W 1-2 |
| 43 | 102 | Jenna WEST | | 44:01. | 8:56. | 5 | W 3 |
| 44 | 31 | Kelsey DEVEREAUX | The Fitness Lab | 44:03. | 8:42. | 5 | W 1-2 |
| 45 | 116 | Risa HUSTAD | Taco Cat Racing | 44:06. | 8:40. | 5 | W 3 |
| 46 | 44 | Kristin COOPER | MadCity Racing | 44:15. | 8:54. | 5 | W 1-2 |
| 47 | 124 | Andrea CYR | Team Velocause | 44:19. | 8:51. | 5 | W 3 |
| 48 | 40 | Sarah BARBER | Sakari Race Team | 44:37. | 9:08. | 5 | W 1-2 |
| 49 | 114 | Amelia VAN MALDEGIAM | PSIMET Racing | 35:37. | 9:39. | 4 | W 3 |
| 50 | 103 | Mary RANDALL | Chicago Cuttin Crew | 35:45. | 8:55. | 4 | W 3 |
