| Rank | Bib | Name | Affiliation | Time | Split Time | Laps | Category |
| 1 | 800 | Melissa SEYMOUR | Team 02 Cadieux | 39:20. | 9:46. | 4 | W 3 |
| 2 | 820 | Allison HUNT | Goldenrods | 39:51. | 9:58. | 4 | W 3 |
| 3 | 801 | Stacey RICHARDSON | Pedal Racing | 41:04. | 9:53. | 4 | W 3 |
| 4 | 807 | Audrey BEEBE | | 41:35. | 10:09. | 4 | W 3 |
| 5 | 808 | Risa HUSTAD | Tacocat Racing | 41:37. | 10:11. | 4 | W 3 |
| 6 | 817 | Ashley BEIMERT | M | 43:08. | 10:57. | 4 | W 3 |
| 7 | 815 | Emily HOESLY | Zealous Racing | 43:35. | 10:52. | 4 | W 3 |
| 8 | 827 | Cassandra KESSLER | University Of Iowa Heart And Va | 43:54. | 11:09. | 4 | W 3 |
| 9 | 804 | Abbey OBRIEN | Trek Midwest Women / Devo Dirt | 43:55. | 10:59. | 4 | W 3 |
| 10 | 805 | Justine PAGENHARDT | Pro Mountain Outfitters | 44:01. | 11:01. | 4 | W 3 |
| 11 | 819 | Joy FISCHER | Battleaxe | 44:32. | 10:57. | 4 | W 3 |
| 12 | 825 | Heidi HARDRATH | Velocause Centraal | 45:14. | 11:37. | 4 | W 3 |
| 13 | 816 | Kristy HENDERSON | Girl Fiend Cycling / Hollywood | 45:33. | 11:25. | 4 | W 3 |
| 14 | 810 | Beth CHRISTIANSEN | Union Cycling | 45:36. | 11:31. | 4 | W 3 |
| 15 | 829 | Kathryn MCDICKEN | | 45:45. | 11:26. | 4 | W 3 |
| 16 | 803 | Amelia VAN MALDEGIAM | Psimet Racing | 48:21. | 13:05. | 4 | W 3 |
| 17 | 823 | Sarah LARSON | Lady Gnar Shredders | 48:49. | 12:21. | 4 | W 3 |
| 18 | 822 | Natalia MENDEZ | The Swendez Collective P/b Savo | 37:47. | 12:56. | 3 | W 3 |
| DNF | 813 | Harper BEASLEY | | | 11:49. | 1 | W 3 |
| DNS | 802 | Annicka CAMPBELL-DOLLAGHAN | Bff Bikes | | 26:23. | 1 | W 3 |
| DNS | 814 | Abby KACENA | Noco Cycling Project | | 26:23. | 1 | W 3 |
| DNS | 806 | Darian NAGLE-GAMM | University Of Iowa Hospitals He | | 26:23. | 1 | W 3 |
| DNS | 821 | Cara HAMANN | Uihvc/iowa City Cycling Club | | 26:23. | 1 | W 3 |
| DNS | 824 | Marnie PEARSALL | Twin Six | | 26:23. | 1 | W 3 |
