| Rank | Bib | Name | Affiliation | Time | Gap | Category |
| 1 | 227 | Tina PIC | Papa Johns Racing p/b TREK | 54:19. | 54:19 | W P12 |
| 2 | 204 | Janelle COLE | UCI WPT: UnitedHealthcare Pro C | 54:20. | 1 | W P12 |
| 3 | 225 | Diana PENUELA | | 54:26. | 7 | W P12 |
| 4 | 236 | Paige SHUMSKAS | Fearless Femme Racing | 54:26. | s.t. | W P12 |
| 5 | 238 | Josie TALBOT | ISCorp Cycling | 54:26. | s.t. | W P12 |
| 6 | 212 | Lauren HALL | UCI WPT: UnitedHealthcare Pro C | 54:27. | s.t. | W P12 |
| 7 | 228 | Laurel RATHBUN | Papa Johns Racing p/b TREK | 54:27. | s.t. | W P12 |
| 8 | 202 | Kyrstin BLUHM | Fearless Femme Racing | 54:27. | s.t. | W P12 |
| 9 | 232 | Jennifer SHARP | Chicago Womens Elite Cycling | 54:27. | s.t. | W P12 |
| 10 | 244 | Lily WILLIAMS | Fearless Femme Racing | 54:27. | s.t. | W P12 |
| 11 | 261 | Kat SWEATT | Mellow Mushroom Racing | 54:27. | s.t. | W P12 |
| 12 | 218 | Rachel LANGDON | ISCorp Powered By Progress | 54:28. | s.t. | W P12 |
| 13 | 249 | Anne DONLEY | | 54:28. | s.t. | W P12 |
| 14 | 205 | Sarah CONEY | | 54:28. | s.t. | W P12 |
| 15 | 233 | Hannah SHELL | Pastaria - Big Shark Racing Tea | 54:28. | s.t. | W P12 |
| 16 | 248 | Flora YAN | Fearless Femme Racing | 54:28. | s.t. | W P12 |
| 17 | 223 | Debbie MILNE | Papa Johns Racing p/b TREK | 54:29. | s.t. | W P12 |
| 18 | 221 | Amy MCCLINTOCK | Pastaria - Big Shark Racing Tea | 54:29. | s.t. | W P12 |
| 19 | 203 | Rachel CANNING | | 54:29. | s.t. | W P12 |
| 20 | 256 | Brenna WRYE-SIMPSON | LA Sweat | 54:29. | s.t. | W P12 |
| 21 | 260 | Melanie BEALE | LA Sweat | 54:29. | s.t. | W P12 |
| 22 | 213 | Lauretta HANSON | | 54:30. | s.t. | W P12 |
| 23 | 252 | Julyn AGUILA | Conade-Visit Mexico-Specialized | 54:30. | s.t. | W P12 |
| 24 | 234 | Katherine SHERWIN | UCI WPT: UnitedHealthcare Pro C | 54:31. | s.t. | W P12 |
| 25 | 206 | Vanessa CURTIS | Orion Racing p/b Kul Chocolate | 54:31. | s.t. | W P12 |
| 26 | 246 | Lauren WISSMAN | Chicago Womens Elite Cycling | 54:31. | s.t. | W P12 |
| 27 | 215 | Daphne KARAGIANIS | Velo Classic p/b Stans NoTubes | 54:31. | s.t. | W P12 |
| 28 | 231 | Carol SEIPP | Orion Racing p/b Kul Chocolate | 54:31. | s.t. | W P12 |
| 29 | 201 | Caroline BAUR | | 54:32. | s.t. | W P12 |
| 30 | 226 | Kimberly PETTIT | Papa Johns Racing p/b TREK | 54:32. | s.t. | W P12 |
| 31 | 251 | Erika VARELA | Conade-Visit Mexico-Specialized | 54:55. | 36 | W P12 |
| 32 | 243 | Ashley WEAVER | Pastaria - Big Shark Racing Tea | 54:56. | s.t. | W P12 |
| 33 | 235 | Tabitha SHERWOOD | Pastaria - Big Shark Racing Tea | 55:10. | 51 | W P12 |
| 34 | 217 | Kate KIRKPATRICK | Mellow Mushroom p/b Psimet | 55:11. | s.t. | W P12 |
| 35 | 255 | Mia CHEESEMAN | Team Wheel & Sprocket | 55:11. | s.t. | W P12 |
| 36 | 250 | Antonieta GAXIOLA | Conade-Visit Mexico-Specialized | 55:12. | s.t. | W P12 |
| 37 | 263 | Carrie CASH WOOTTEN | Team Revolution | 55:12. | s.t. | W P12 |
| 38 | 242 | Jennifer WAGNER | Mellow Mushroom p/b Psimet | 55:12. | s.t. | W P12 |
| 39 | 222 | Nicole MERTZ | Velo Classic p/b Stans NoTubes | 56:31. | 2:12 | W P12 |
| 40 | 259 | Christa GHENT | LA Sweat | 57:03. | 2:44 | W P12 |
| 41 | 257 | Tiffany PEZZULO | LA Sweat | 57:09. | 2:50 | W P12 |
| 42 | 245 | Rachel WILLS | Chicago Womens Elite Cycling | 57:22. | 3:03 | W P12 |
| 43 | 220 | Lauren LECLAIRE | Pedal Power Team-MA | 57:22. | s.t. | W P12 |
| 44 | 239 | Annika TESCHKE | | 57:22. | s.t. | W P12 |
| 45 | 210 | Sarah GUILBERT | RTO National Womens Cycling | 57:22. | s.t. | W P12 |
| 46 | 211 | Jennifer HALE | Orion Racing p/b Kul Chocolate | 57:22. | s.t. | W P12 |
| DNF | 200 | Kristen ARNOLD | Velo Classic p/b Stans NoTubes | | | W P12 |
| DNF | 208 | Cynthia FRAZIER | Velo Classic p/b Stans NoTubes | | | W P12 |
| DNF | 240 | Christine THORNBURG | Chicago Womens Elite Cycling | | | W P12 |
| DNF | 241 | Laura VAN GILDER | Mellow Mushroom p/b Psimet | | | W P12 |
