| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 230 | Kendall RYAN | UCI WPT: Team TIBCO-Silicon Val | 54:39. | 54:39 |
| 2 | 232 | Jennifer SHARP | Chicago Womens Elite Cycling | 54:40. | s.t. |
| 3 | 221 | Amy MCCLINTOCK | Pastaria - Big Shark Racing Tea | 54:40. | s.t. |
| 4 | 241 | Laura VAN GILDER | Mellow Mushroom p/b Psimet | 54:40. | s.t. |
| 5 | 213 | Lauretta HANSON | | 54:40. | s.t. |
| 6 | 238 | Josie TALBOT | ISCorp Cycling | 54:40. | s.t. |
| 7 | 227 | Tina PIC | Papa Johns Racing p/b TREK | 54:40. | s.t. |
| 8 | 205 | Sarah CONEY | | 54:40. | s.t. |
| 9 | 251 | Erika VARELA | Conade-Visit Mexico-Specialized | 54:40. | s.t. |
| 10 | 228 | Laurel RATHBUN | Papa Johns Racing p/b TREK | 54:41. | s.t. |
| 11 | 218 | Rachel LANGDON | ISCorp Powered By Progress | 54:41. | s.t. |
| 12 | 244 | Lily WILLIAMS | Fearless Femme Racing | 54:41. | s.t. |
| 13 | 209 | Danielydelvalle GARCIA | | 54:41. | s.t. |
| 14 | 225 | Diana PENUELA | | 54:41. | s.t. |
| 15 | 212 | Lauren HALL | UCI WPT: UnitedHealthcare Pro C | 54:42. | s.t. |
| 16 | 202 | Kyrstin BLUHM | Fearless Femme Racing | 54:42. | s.t. |
| 17 | 257 | Tiffany PEZZULO | LA Sweat | 54:42. | s.t. |
| 18 | 223 | Debbie MILNE | Papa Johns Racing p/b TREK | 54:42. | s.t. |
| 19 | 204 | Janelle COLE | UCI WPT: UnitedHealthcare Pro C | 54:42. | s.t. |
| 20 | 259 | Christa GHENT | LA Sweat | 54:42. | s.t. |
| 21 | 233 | Hannah SHELL | Pastaria - Big Shark Racing Tea | 54:43. | s.t. |
| 22 | 261 | Kat SWEATT | Mellow Mushroom Racing | 54:43. | s.t. |
| 23 | 242 | Jennifer WAGNER | Mellow Mushroom p/b Psimet | 54:43. | s.t. |
| 24 | 201 | Caroline BAUR | | 54:43. | s.t. |
| 25 | 235 | Tabitha SHERWOOD | Pastaria - Big Shark Racing Tea | 54:43. | s.t. |
| 26 | 210 | Sarah GUILBERT | RTO National Womens Cycling | 54:43. | s.t. |
| 27 | 237 | Britta SIEGEL | | 54:43. | s.t. |
| 28 | 206 | Vanessa CURTIS | Orion Racing p/b Kul Chocolate | 54:44. | s.t. |
| 29 | 217 | Kate KIRKPATRICK | Mellow Mushroom p/b Psimet | 54:44. | s.t. |
| 30 | 239 | Annika TESCHKE | | 54:44. | s.t. |
| 31 | 214 | Elizabeth HARDEN | Papa Johns Racing p/b TREK | 54:52. | 13 |
| 32 | 211 | Jennifer HALE | Orion Racing p/b Kul Chocolate | 54:54. | 15 |
| 33 | 252 | Julyn AGUILA | Conade-Visit Mexico-Specialized | 54:55. | s.t. |
| 34 | 246 | Lauren WISSMAN | Chicago Womens Elite Cycling | 54:55. | s.t. |
| 35 | 226 | Kimberly PETTIT | Papa Johns Racing p/b TREK | 54:55. | s.t. |
| 36 | 240 | Christine THORNBURG | Chicago Womens Elite Cycling | 54:56. | s.t. |
| 37 | 215 | Daphne KARAGIANIS | Velo Classic p/b Stans NoTubes | 54:58. | 19 |
| 38 | 253 | Veronica LEAL | | 54:58. | s.t. |
| 39 | 250 | Antonieta GAXIOLA | Conade-Visit Mexico-Specialized | 55:00. | 21 |
| 40 | 248 | Flora YAN | Fearless Femme Racing | 55:00. | s.t. |
| 41 | 220 | Lauren LECLAIRE | Pedal Power Team-MA | 55:06. | 27 |
| 42 | 203 | Rachel CANNING | | 55:06. | s.t. |
| 43 | 231 | Carol SEIPP | Orion Racing p/b Kul Chocolate | 55:06. | s.t. |
| 44 | 222 | Nicole MERTZ | Velo Classic p/b Stans NoTubes | 55:24. | 45 |
| 45 | 260 | Melanie BEALE | LA Sweat | 55:30. | 51 |
| 46 | 254 | Jennifer SCHOOK | Midwest Cycling Trek Stores Wom | 55:45. | 1:06 |
| 47 | 256 | Brenna WRYE-SIMPSON | LA Sweat | 55:47. | 1:08 |
| 48 | 249 | Anne DONLEY | | 58:36. | 3:57 |
| 49 | 234 | Katherine SHERWIN | UCI WPT: UnitedHealthcare Pro C | 58:36. | s.t. |
| 50 | 243 | Ashley WEAVER | Pastaria - Big Shark Racing Tea | 58:36. | s.t. |
| 51 | 207 | Mary Emily DAVIDSON | Pursuit Cycling | 58:36. | s.t. |
| 52 | 247 | Nina WOLLASTON | | 58:36. | s.t. |
| 53 | 245 | Rachel WILLS | Chicago Womens Elite Cycling | 58:36. | s.t. |
| 54 | 219 | Maria LARKIN | Chicago Womens Elite Cycling | 58:36. | s.t. |
| DQ | 255 | Mia CHEESEMAN | Team Wheel & Sprocket | | |
