| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 2 | Erica ALLAR | UCI WPT: Rally Cycling | 1:16:13. | 1:16:13 |
| 2 | 34 | Lauren STEPHENS | UCI WPT: Team TIBCO-Silicon Val | 1:16:14. | s.t. |
| 3 | 27 | Irena OSSOLA | SAS-MACOGEP | 1:16:19. | 6 |
| 4 | 31 | Skylar SCHNEIDER * | ISCorp Powered By Progress | 1:16:36. | 23 |
| 5 | 29 | Kendall RYAN | UCI WPT: Team TIBCO-Silicon Val | 1:16:36. | s.t. |
| 6 | 43 | Emma WHITE * | UCI WPT: Rally Cycling | 1:16:36. | s.t. |
| 7 | 50 | Tina PIC | Papa Johns Racing p/b TREK | 1:16:37. | s.t. |
| 8 | 55 | Laura VAN GILDER | Mellow Mushroom p/b Psimet | 1:16:38. | s.t. |
| 9 | 32 | Hannah SHELL | Pastaria - Big Shark Racing Tea | 1:16:38. | s.t. |
| 10 | 12 | Heather FISCHER | UCI WPT: Team TIBCO-Silicon Val | 1:16:38. | s.t. |
| 11 | 54 | Kat SWEATT | Mellow Mushroom Racing | 1:16:39. | s.t. |
| 12 | 44 | Lily WILLIAMS | Chicago Womens Elite Cycling | 1:16:39. | s.t. |
| 13 | 17 | Laura JORGENSEN | ~ | 1:16:39. | s.t. |
| 14 | 48 | Payten MANESS * | Papa Johns Racing p/b TREK | 1:16:39. | s.t. |
| 15 | 13 | Cynthia FRAZIER | Velo Classic p/b Stans NoTubes | 1:16:39. | s.t. |
| 16 | 33 | Tabitha SHERWOOD | Pastaria - Big Shark Racing Tea | 1:16:40. | s.t. |
| 17 | 24 | Danielle MORSHEAD * | Amy D. Foundation | 1:16:40. | s.t. |
| 18 | 16 | Megan JASTRAB * | Swamis Junior Development Team | 1:16:40. | s.t. |
| 19 | 56 | Christy KEELY | TotalCyclist | 1:16:40. | s.t. |
| 20 | 11 | Ashley DUBAN | ATX HitSquad | 1:16:41. | s.t. |
| 21 | 47 | Madison KELLY | Papa Johns Racing p/b TREK | 1:16:41. | s.t. |
| 22 | 22 | Nicole MERTZ | Velo Classic p/b Stans NoTubes | 1:16:42. | s.t. |
| 23 | 51 | Laurel RATHBUN * | Papa Johns Racing p/b TREK | 1:16:42. | s.t. |
| 24 | 41 | Brianna WALLE | UCI WPT: Team TIBCO-Silicon Val | 1:16:42. | s.t. |
| 25 | 39 | Monica VOLK | UCI WPT: Rally Cycling | 1:16:43. | s.t. |
| 26 | 46 | Lauren WISSMAN | Chicago Womens Elite Cycling | 1:16:43. | s.t. |
| 27 | 40 | Elizabeth HARDEN * | RTO National Cycling | 1:16:44. | s.t. |
| 28 | 23 | Marta MORRIS * | Stages Cycling | 1:16:44. | s.t. |
| 29 | 45 | Rachel WILLS | Chicago Womens Elite Cycling | 1:16:44. | s.t. |
| 30 | 15 | Megan HEATH * | Rally Cycling ""Devo"" | 1:16:47. | 34 |
| 31 | 3 | Kristen ARNOLD | Velo Classic p/b Stans NoTubes | 1:16:48. | s.t. |
| 32 | 36 | Christine THORNBURG | Chicago Womens Elite Cycling | 1:16:50. | 37 |
| 33 | 35 | Jennifer TETRICK | UCI WPT: Team TIBCO-Silicon Val | 1:16:54. | 41 |
| 34 | 42 | Ashley WEAVER | Pastaria - Big Shark Racing Tea | 1:16:58. | 45 |
| 35 | 52 | Paige SHUMSKAS * | Fearless Femme Racing | 1:17:00. | 47 |
| 36 | 4 | Christina GOKEY-SMITH | Papa Johns Racing p/b TREK | 1:17:04. | 51 |
| 37 | 30 | Samantha SCHNEIDER | ISCorp Powered By Progress | 1:17:34. | 1:21 |
| 38 | 18 | Victoria KANIZER * | OrthoCarolina Winston Women???s | 1:19:00. | 2:47 |
| 39 | 9 | Kelsey DEVEREAUX | WRP | 1:19:01. | s.t. |
| 40 | 53 | Stephanie NAVE | OrthoCarolina Winston Women???s | 1:19:01. | s.t. |
| 41 | 37 | Leslie TIMM | Sunapee Racing Team | 1:19:07. | 2:54 |
| 42 | 10 | Lauren DODGE | WV Zeeuws-Vlaanderen | 1:19:07. | s.t. |
| 43 | 20 | Jennifer KOSATKA | Chicago Womens Elite Cycling | 1:19:07. | s.t. |
| 44 | 38 | Eleanor VELEZ * | ~ | 1:19:07. | s.t. |
| 45 | 5 | Charlotte BACKUS * | Prestige Imports/Porsche/Audi R | 1:19:07. | s.t. |
| DNF | 28 | Mary PENTA | WRP | | |
| DNF | 21 | Caitlin LAROCHE | UCI WPT: Rally Cycling | | |
| DNF | 6 | Amy BENNER | UCI WPT: Rally Cycling | | |
| DNF | 14 | Sarah GUILBERT | RTO National Womens Cycling | | |
