| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 28 | Ruth Winder | UnitedHealthcare Pro Cycling | 2:36:23. | 2:36:23 |
| 2 | 18 | *Emma White | Rally Cycling | 2:36:24. | s.t. |
| 3 | 41 | Marlies MejiasGarcia | Weber Shimano Ladies Power | 2:36:24. | s.t. |
| 4 | 1 | Brianna Walle | Team TIBCO-Silicon Valley Bank | 2:36:24. | s.t. |
| 5 | 21 | Rushlee Buchanan | UnitedHealthcare Pro Cycling | 2:36:26. | 3 |
| 6 | 66 | #Lily Williams | Chicago Womens Elite Cycling | 2:36:26. | s.t. |
| 7 | 71 | *#Ariane Bonhomme | The Cyclery - 4iiii | 2:36:26. | s.t. |
| 8 | 14 | Allison Beveridge | Rally Cycling | 2:36:27. | s.t. |
| 9 | 27 | Tayler Wiles | UnitedHealthcare Pro Cycling | 2:36:28. | 5 |
| 10 | 81 | #Ashlee Ankudinoff | North Memorial Healthcare | 2:36:29. | s.t. |
| 11 | 32 | Emma Grant | Colavita/Bianchi USA | 2:36:31. | 8 |
| 12 | 17 | *Katherine Maine | Rally Cycling | 2:36:39. | 16 |
| 13 | 53 | #Tarah Cole | Orion Racing p/b Kul Chocolate | 2:36:40. | s.t. |
| 14 | 11 | *Sara Poidevin | Rally Cycling | 2:36:41. | 18 |
| 15 | 13 | Sara Bergen | Rally Cycling | 2:36:45. | 22 |
| 16 | 31 | Whitney Allison | Colavita/Bianchi USA | 2:36:46. | s.t. |
| 17 | 12 | Erica Allar | Rally Cycling | 2:36:57. | 34 |
| 18 | 4 | Heather Fischer | Team TIBCO-Silicon Valley Bank | 2:36:57. | s.t. |
| 19 | 141 | *#Danielle Morshead | Team ERRACE | 2:36:58. | s.t. |
| 20 | 86 | Irena Ossola | North Memorial Healthcare | 2:36:58. | s.t. |
| 21 | 24 | Katharine Hall | UnitedHealthcare Pro Cycling | 2:36:58. | s.t. |
| 22 | 78 | Stephanie Roorda | The Cyclery - 4iiii | 2:36:58. | s.t. |
| 23 | 105 | #Brenna Wrye-Simpson | LA Sweat | 2:36:58. | s.t. |
| 24 | 74 | Annie Foreman-Mackey | The Cyclery - 4iiii | 2:36:58. | s.t. |
| 25 | 44 | *Camila Valbuena | Weber Shimano Ladies Power | 2:37:00. | 37 |
| 26 | 16 | Kirsti Lay | Rally Cycling | 2:37:01. | 38 |
| 27 | 93 | Elyse Fraser | Cycling New Zealand | 2:37:06. | 43 |
| 28 | 111 | #Carrie Cartmill | Rise Racing | 2:37:19. | 56 |
| 29 | 95 | Racquel Sheath | Cycling New Zealand | 2:37:22. | 59 |
| 30 | 43 | Rocio Parrado | Weber Shimano Ladies Power | 2:37:24. | 1:01 |
| 31 | 92 | *Holly Edmondston | Cycling New Zealand | 2:37:32. | 1:09 |
| 32 | 26 | Diana Penuela | UnitedHealthcare Pro Cycling | 2:38:04. | 1:41 |
| 33 | 22 | *Janelle Cole | UnitedHealthcare Pro Cycling | 2:38:05. | s.t. |
| 34 | 37 | Amber Pierce | Colavita/Bianchi USA | 2:38:05. | s.t. |
| 35 | 55 | #Carlyn Jackson | Orion Racing p/b Kul Chocolate | 2:38:20. | 1:57 |
| 36 | 85 | Beth Ann Orton | North Memorial Healthcare | 2:38:40. | 2:17 |
| 37 | 77 | *Catherine Ouellette | The Cyclery - 4iiii | 2:39:30. | 3:07 |
| 38 | 102 | Anna Christiansen | LA Sweat | 2:39:33. | 3:10 |
| 39 | 82 | Marie-Soleil Blais | North Memorial Healthcare | 2:39:34. | 3:11 |
| 40 | 8 | Jennifer Tetrick | Team TIBCO-Silicon Valley Bank | 2:39:43. | 3:20 |
| 41 | 7 | Kendall Ryan | Team TIBCO-Silicon Valley Bank | 2:39:43. | s.t. |
| 42 | 6 | *Madeleine Park | Team TIBCO-Silicon Valley Bank | 2:39:43. | s.t. |
| 43 | 62 | #Heather Haviland | Chicago Womens Elite Cycling | 2:39:43. | s.t. |
| 44 | 33 | Kendelle Hodges | Colavita/Bianchi USA | 2:39:43. | s.t. |
| 45 | 35 | Jessica Mundy | Colavita/Bianchi USA | 2:39:43. | s.t. |
| 46 | 45 | *Aranza Villalon | Weber Shimano Ladies Power | 2:39:44. | s.t. |
| 47 | 36 | *Ellen Noble | Colavita/Bianchi USA | 2:39:44. | s.t. |
| 48 | 15 | Caitlin Laroche | Rally Cycling | 2:40:13. | 3:50 |
| 49 | 104 | #Kimberly Lucie | LA Sweat | 2:41:22. | 4:59 |
| 50 | 83 | #Jennifer Hale | North Memorial Healthcare | 2:41:33. | 5:10 |
| 51 | 56 | #Emily Jordan | Orion Racing p/b Kul Chocolate | 2:41:34. | 5:11 |
| 52 | 91 | *Bryony Botha | Cycling New Zealand | 2:41:43. | 5:20 |
| 53 | 75 | *#Kinley Gibson | The Cyclery - 4iiii | 2:41:43. | s.t. |
| 54 | 68 | #Lauren Wissman | Chicago Womens Elite Cycling | 2:41:56. | 5:33 |
| 55 | 94 | Kirstie James | Cycling New Zealand | 2:42:10. | 5:47 |
| 56 | 52 | *#Alijah Beatty | Orion Racing p/b Kul Chocolate | 2:42:10. | s.t. |
| 57 | 42 | *Jessenia Meneses | Weber Shimano Ladies Power | 2:42:12. | 5:49 |
| 58 | 46 | Maria Fadiga | Weber Shimano Ladies Power | 2:42:12. | s.t. |
| 51 | #Diedre Ribbens | Orion Racing p/b Kul Chocolate | | |
| 58 | #Jenna Stauffer | Orion Racing p/b Kul Chocolate | | |
| 65 | #Christine Thornburg | Chicago Womens Elite Cycling | | |
| 67 | #Rachel Wills | Chicago Womens Elite Cycling | | |
| 72 | #Am?lie Bruneau | The Cyclery - 4iiii | | |
| 73 | #Emily Flynn | The Cyclery - 4iiii | | |
| 76 | #Suzanne Hamilton | The Cyclery - 4iiii | | |
| 84 | *#Nicola Macdonald | North Memorial Healthcare | | |
| 103 | #Erin Goodall | LA Sweat | | |
| 121 | #Ayesha McGowan | JAKROO p/b Chrome Industries | | |
| 131 | #Rachel McKinnon | Foxy Moxy | | |
