| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 18 | *Emma White | Rally Cycling | 56:46. | 56:46 |
| 2 | 41 | Marlies MejiasGarcia | Weber Shimano Ladies Power | 56:46. | s.t. |
| 3 | 36 | *Ellen Noble | Colavita/Bianchi USA | 56:46. | s.t. |
| 4 | 7 | Kendall Ryan | Team TIBCO-Silicon Valley Bank | 56:47. | s.t. |
| 5 | 12 | Erica Allar | Rally Cycling | 56:47. | s.t. |
| 6 | 86 | Irena Ossola | North Memorial Healthcare | 56:49. | 3 |
| 7 | 14 | Allison Beveridge | Rally Cycling | 56:49. | s.t. |
| 8 | 78 | Stephanie Roorda | The Cyclery - 4iiii | 56:49. | s.t. |
| 9 | 104 | #Kimberly Lucie | LA Sweat | 56:50. | s.t. |
| 10 | 111 | #Carrie Cartmill | Rise Racing | 56:50. | s.t. |
| 11 | 105 | #Brenna Wrye-Simpson | LA Sweat | 56:50. | s.t. |
| 12 | 66 | #Lily Williams | Chicago Womens Elite Cycling | 56:50. | s.t. |
| 13 | 85 | Beth Ann Orton | North Memorial Healthcare | 56:50. | s.t. |
| 14 | 1 | Brianna Walle | Team TIBCO-Silicon Valley Bank | 56:51. | s.t. |
| 15 | 44 | *Camila Valbuena | Weber Shimano Ladies Power | 56:51. | s.t. |
| 16 | 42 | *Jessenia Meneses | Weber Shimano Ladies Power | 56:52. | s.t. |
| 17 | 26 | Diana Penuela | UnitedHealthcare Pro Cycling | 56:53. | s.t. |
| 18 | 141 | *#Danielle Morshead | Team ERRACE | 56:54. | s.t. |
| 19 | 71 | *#Ariane Bonhomme | The Cyclery - 4iiii | 56:54. | s.t. |
| 20 | 37 | Amber Pierce | Colavita/Bianchi USA | 56:54. | s.t. |
| 21 | 11 | *Sara Poidevin | Rally Cycling | 56:54. | s.t. |
| 22 | 15 | Caitlin Laroche | Rally Cycling | 56:54. | s.t. |
| 23 | 82 | Marie-Soleil Blais | North Memorial Healthcare | 56:55. | s.t. |
| 24 | 84 | *#Nicola Macdonald | North Memorial Healthcare | 56:55. | s.t. |
| 25 | 43 | Rocio Parrado | Weber Shimano Ladies Power | 56:55. | s.t. |
| 26 | 52 | *#Alijah Beatty | Orion Racing p/b Kul Chocolate | 56:55. | s.t. |
| 27 | 95 | Racquel Sheath | Cycling New Zealand | 56:55. | s.t. |
| 28 | 13 | Sara Bergen | Rally Cycling | 56:56. | s.t. |
| 29 | 92 | *Holly Edmondston | Cycling New Zealand | 56:56. | s.t. |
| 30 | 31 | Whitney Allison | Colavita/Bianchi USA | 56:56. | s.t. |
| 31 | 27 | Tayler Wiles | UnitedHealthcare Pro Cycling | 56:56. | s.t. |
| 32 | 46 | Maria Fadiga | Weber Shimano Ladies Power | 56:56. | s.t. |
| 33 | 22 | *Janelle Cole | UnitedHealthcare Pro Cycling | 56:56. | s.t. |
| 34 | 93 | Elyse Fraser | Cycling New Zealand | 56:57. | s.t. |
| 35 | 6 | *Madeleine Park | Team TIBCO-Silicon Valley Bank | 56:57. | s.t. |
| 36 | 81 | #Ashlee Ankudinoff | North Memorial Healthcare | 56:57. | s.t. |
| 37 | 32 | Emma Grant | Colavita/Bianchi USA | 57:01. | 15 |
| 38 | 55 | #Carlyn Jackson | Orion Racing p/b Kul Chocolate | 57:01. | s.t. |
| 39 | 35 | Jessica Mundy | Colavita/Bianchi USA | 57:01. | s.t. |
| 40 | 68 | #Lauren Wissman | Chicago Womens Elite Cycling | 57:02. | s.t. |
| 41 | 77 | *Catherine Ouellette | The Cyclery - 4iiii | 57:02. | s.t. |
| 42 | 17 | *Katherine Maine | Rally Cycling | 57:02. | s.t. |
| 43 | 102 | #Anna Christiansen | LA Sweat | 57:02. | s.t. |
| 44 | 4 | Heather Fischer | Team TIBCO-Silicon Valley Bank | 57:03. | s.t. |
| 45 | 57 | #Carol Seipp | Orion Racing p/b Kul Chocolate | 57:03. | s.t. |
| 46 | 121 | #Ayesha McGowan | JAKROO p/b Chrome Industries | 57:04. | 18 |
| 47 | 62 | #Heather Haviland | Chicago Womens Elite Cycling | 57:04. | s.t. |
| 48 | 67 | #Rachel Wills | Chicago Womens Elite Cycling | 57:05. | s.t. |
| 49 | 74 | Annie Foreman-Mackey | The Cyclery - 4iiii | 57:06. | s.t. |
| 50 | 16 | Kirsti Lay | Rally Cycling | 57:07. | s.t. |
| 51 | 33 | Kendelle Hodges | Colavita/Bianchi USA | 57:07. | s.t. |
| 52 | 51 | #Diedre Ribbens | Orion Racing p/b Kul Chocolate | 57:13. | 27 |
| 53 | 64 | #Jennifer Kosatka | Chicago Womens Elite Cycling | 57:16. | 30 |
| 54 | 54 | #Emily Houtchens | Orion Racing p/b Kul Chocolate | 57:17. | s.t. |
| 55 | 101 | #Melanie Beale | LA Sweat | 57:17. | s.t. |
| 56 | 53 | #Tarah Cole | Orion Racing p/b Kul Chocolate | 57:17. | s.t. |
| 57 | 83 | #Jennifer Hale | North Memorial Healthcare | 57:18. | s.t. |
| 58 | 56 | #Emily Jordan | Orion Racing p/b Kul Chocolate | 57:19. | s.t. |
| 59 | 65 | #Christine Thornburg | Chicago Womens Elite Cycling | 57:19. | s.t. |
| 60 | 72 | #Am?lie Bruneau | The Cyclery - 4iiii | 57:20. | s.t. |
| 61 | 45 | *Aranza Villalon | Weber Shimano Ladies Power | 57:20. | s.t. |
| 62 | 75 | *#Kinley Gibson | The Cyclery - 4iiii | 57:21. | s.t. |
| 63 | 103 | #Erin Goodall | LA Sweat | 57:22. | 36 |
| 64 | 73 | #Emily Flynn | The Cyclery - 4iiii | 57:31. | 45 |
| 65 | 76 | #Suzanne Hamilton | The Cyclery - 4iiii | 57:32. | s.t. |
| 66 | 58 | #Jenna Stauffer | Orion Racing p/b Kul Chocolate | 57:32. | s.t. |
| 67 | 131 | #Rachel McKinnon | Foxy Moxy | 57:45. | 59 |
| 68 | 87 | #Emma Roberts | North Memorial Healthcare | 57:48. | 1:02 |
| 69 | 91 | *Bryony Botha | Cycling New Zealand | 58:23. | 1:37 |
| 70 | 5 | Lindsay Myers | Team TIBCO-Silicon Valley Bank | 58:58. | 2:12 |
| 3 | Ingrid Drexel Clouthier | Team TIBCO-Silicon Valley Bank | | |
| 28 | Ruth Winder | UnitedHealthcare Pro Cycling | | |
| 21 | Rushlee Buchanan | UnitedHealthcare Pro Cycling | | |
| 2 | Kathryn Buss | Team TIBCO-Silicon Valley Bank | | |
| 8 | Jennifer Tetrick | Team TIBCO-Silicon Valley Bank | | |
| 24 | Katharine Hall | UnitedHealthcare Pro Cycling | | |
| 25 | Lauretta Hanson | UnitedHealthcare Pro Cycling | | |
| 61 | #Annie Byrne | Chicago Womens Elite Cycling | | |
| 94 | Kirstie James | Cycling New Zealand | | |
