| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 12 | Erica Allar | Rally Cycling | 16:44. | 16:44 |
| 2 | 35 | Jessica Mundy | Colavita/Bianchi USA | 16:45. | s.t. |
| 3 | 86 | Irena Ossola | North Memorial Healthcare | 16:45. | s.t. |
| 7 | Kendall Ryan | Team TIBCO-Silicon Valley Bank | | |
| 28 | Ruth Winder | UnitedHealthcare Pro Cycling | | |
| 21 | Rushlee Buchanan | UnitedHealthcare Pro Cycling | | |
| 1 | Brianna Walle | Team TIBCO-Silicon Valley Bank | | |
| 2 | Kathryn Buss | Team TIBCO-Silicon Valley Bank | | |
| 3 | Ingrid Drexel Clouthier | Team TIBCO-Silicon Valley Bank | | |
| 4 | Heather Fischer | Team TIBCO-Silicon Valley Bank | | |
| 5 | Lindsay Myers | Team TIBCO-Silicon Valley Bank | | |
| 6 | *Madeleine Park | Team TIBCO-Silicon Valley Bank | | |
| 8 | Jennifer Tetrick | Team TIBCO-Silicon Valley Bank | | |
| 11 | *Sara Poidevin | Rally Cycling | | |
| 13 | Sara Bergen | Rally Cycling | | |
| 14 | Allison Beveridge | Rally Cycling | | |
| 15 | Caitlin Laroche | Rally Cycling | | |
| 16 | Kirsti Lay | Rally Cycling | | |
| 17 | *Katherine Maine | Rally Cycling | | |
| 18 | *Emma White | Rally Cycling | | |
| 22 | *Janelle Cole | UnitedHealthcare Pro Cycling | | |
| 24 | Katharine Hall | UnitedHealthcare Pro Cycling | | |
| 25 | Lauretta Hanson | UnitedHealthcare Pro Cycling | | |
| 26 | Diana Penuela | UnitedHealthcare Pro Cycling | | |
| 27 | Tayler Wiles | UnitedHealthcare Pro Cycling | | |
| 31 | Whitney Allison | Colavita/Bianchi USA | | |
| 32 | Emma Grant | Colavita/Bianchi USA | | |
| 33 | Kendelle Hodges | Colavita/Bianchi USA | | |
| 36 | *Ellen Noble | Colavita/Bianchi USA | | |
| 37 | Amber Pierce | Colavita/Bianchi USA | | |
| 41 | Marlies MejiasGarcia | Weber Shimano Ladies Power | | |
| 42 | *Jessenia Meneses | Weber Shimano Ladies Power | | |
| 43 | Rocio Parrado | Weber Shimano Ladies Power | | |
| 44 | *Camila Valbuena | Weber Shimano Ladies Power | | |
| 45 | *Aranza Villalon | Weber Shimano Ladies Power | | |
| 46 | Maria Fadiga | Weber Shimano Ladies Power | | |
| 51 | #Diedre Ribbens | Orion Racing p/b Kul Chocolate | | |
| 52 | *#Alijah Beatty | Orion Racing p/b Kul Chocolate | | |
| 53 | #Tarah Cole | Orion Racing p/b Kul Chocolate | | |
| 54 | #Emily Houtchens | Orion Racing p/b Kul Chocolate | | |
| 55 | #Carlyn Jackson | Orion Racing p/b Kul Chocolate | | |
| 56 | #Emily Jordan | Orion Racing p/b Kul Chocolate | | |
| 57 | #Carol Seipp | Orion Racing p/b Kul Chocolate | | |
| 58 | #Jenna Stauffer | Orion Racing p/b Kul Chocolate | | |
| 61 | #Annie Byrne | Chicago Womens Elite Cycling | | |
| 62 | #Heather Haviland | Chicago Womens Elite Cycling | | |
| 64 | #Jennifer Kosatka | Chicago Womens Elite Cycling | | |
| 65 | #Christine Thornburg | Chicago Womens Elite Cycling | | |
| 66 | #Lily Williams | Chicago Womens Elite Cycling | | |
| 67 | #Rachel Wills | Chicago Womens Elite Cycling | | |
| 68 | #Lauren Wissman | Chicago Womens Elite Cycling | | |
| 71 | *#Ariane Bonhomme | The Cyclery - 4iiii | | |
| 72 | #Am?lie Bruneau | The Cyclery - 4iiii | | |
| 73 | #Emily Flynn | The Cyclery - 4iiii | | |
| 74 | Annie Foreman-Mackey | The Cyclery - 4iiii | | |
| 75 | *#Kinley Gibson | The Cyclery - 4iiii | | |
| 76 | #Suzanne Hamilton | The Cyclery - 4iiii | | |
| 77 | *Catherine Ouellette | The Cyclery - 4iiii | | |
| 78 | Stephanie Roorda | The Cyclery - 4iiii | | |
| 81 | #Ashlee Ankudinoff | North Memorial Healthcare | | |
| 82 | Marie-Soleil Blais | North Memorial Healthcare | | |
| 83 | #Jennifer Hale | North Memorial Healthcare | | |
| 84 | *#Nicola Macdonald | North Memorial Healthcare | | |
| 85 | Beth Ann Orton | North Memorial Healthcare | | |
| 87 | #Emma Roberts | North Memorial Healthcare | | |
| 91 | *Bryony Botha | Cycling New Zealand | | |
| 92 | *Holly Edmondston | Cycling New Zealand | | |
| 93 | Elyse Fraser | Cycling New Zealand | | |
| 94 | Kirstie James | Cycling New Zealand | | |
| 95 | Racquel Sheath | Cycling New Zealand | | |
| 101 | #Melanie Beale | LA Sweat | | |
| 102 | #Anna Christiansen | LA Sweat | | |
| 103 | #Erin Goodall | LA Sweat | | |
| 104 | #Kimberly Lucie | LA Sweat | | |
| 105 | #Brenna Wrye-Simpson | LA Sweat | | |
| 111 | #Carrie Cartmill | Rise Racing | | |
| 121 | #Ayesha McGowan | JAKROO p/b Chrome Industries | | |
| 131 | #Rachel McKinnon | Foxy Moxy | | |
| 141 | *#Danielle Morshead | Team ERRACE | | |
