| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 192 | Lauren HALL | UCI WPT: UnitedHealthcare Pro C | 1:02:31. | 1:02:31 |
| 2 | 167 | Erica ALLAR | UCI WPT: Rally Cycling | 1:02:31. | s.t. |
| 3 | 197 | Kendall RYAN | UCI WPT: Team TIBCO-Silicon Val | 1:02:31. | s.t. |
| 4 | 196 | Diana PENUELA | UCI WPT: UnitedHealthcare Pro C | 1:02:31. | s.t. |
| 5 | 174 | Peta MULLENS | UCI WPT: Hagens Berman | Superm | 1:02:32. | s.t. |
| 6 | 191 | Ruth WINDER | UCI WPT: UnitedHealthcare Pro C | 1:02:32. | s.t. |
| 7 | 179 | Mandy HEINTZ | UCI WPT: Visit Dallas DNA Pro C | 1:02:33. | s.t. |
| 8 | 176 | Samantha SCHNEIDER | | 1:02:33. | s.t. |
| 9 | 208 | Jamie GILGEN | Rise Racing | 1:02:33. | s.t. |
| 10 | 173 | Elizabeth WILLIAMS | UCI WPT: Hagens Berman | Superm | 1:02:33. | s.t. |
| 11 | 152 | Christy KEELY | | 1:02:33. | s.t. |
| 12 | 186 | Debbie MILNE | Papa Johns Racing pb TREK | 1:02:34. | s.t. |
| 13 | 185 | Tina PIC | Papa Johns Racing pb TREK | 1:02:37. | 6 |
| 14 | 165 | Hannah ARENSMAN | TWENTY20 Pro Cycling | 1:02:37. | s.t. |
| 15 | 194 | Janelle COLE | UCI WPT: UnitedHealthcare Pro C | 1:02:37. | s.t. |
| 16 | 158 | Kat SWEATT | SH!FT Racing pb St. Vincents O | 1:02:37. | s.t. |
| 17 | 202 | Daphne KARAGIANIS | Velo Classic pb Stans NoTubes | 1:02:38. | 7 |
| 18 | 214 | Tabitha SHERWOOD | Pastaria - Big Shark Racing Tea | 1:02:38. | s.t. |
| 19 | 206 | Miriam BROUWER | Rise Racing | 1:02:39. | s.t. |
| 20 | 201 | Erica ZAVETA | UCI WPT: Cylance Pro Cycling | 1:02:39. | s.t. |
| 21 | 221 | Laura VAN GILDER | Mellow Mushroom pb Psimet | 1:02:39. | s.t. |
| 22 | 178 | Rachel LANGDON | | 1:02:39. | s.t. |
| 23 | 177 | Skylar SCHNEIDER | | 1:02:39. | s.t. |
| 24 | 155 | Laura JORGENSEN | | 1:02:39. | s.t. |
| 25 | 215 | Hannah SHELL | Pastaria - Big Shark Racing Tea | 1:02:40. | s.t. |
| 26 | 187 | Madison KELLY | Papa Johns Racing pb TREK | 1:02:40. | s.t. |
| 27 | 212 | Arden STELLY | OrthoCarolina Winston Womens C | 1:02:41. | s.t. |
| 28 | 216 | Ashley WEAVER | Pastaria - Big Shark Racing Tea | 1:02:41. | s.t. |
| 29 | 207 | Karlee GENDRON | Rise Racing | 1:02:41. | s.t. |
| 30 | 222 | Kate KIRKPATRICK | Mellow Mushroom pb Psimet | 1:02:42. | s.t. |
| 31 | 190 | Laurel RATHBUN | Papa Johns Racing pb TREK | 1:02:42. | s.t. |
| 32 | 164 | Judah SENCENBAUGH | CTH Performance Project | 1:02:44. | 13 |
| 33 | 180 | Hanna MUEGGE | UCI WPT: Visit Dallas DNA Pro C | 1:02:45. | 14 |
| 34 | 210 | Stephanie NAVE | OrthoCarolina Winston Womens C | 1:02:46. | s.t. |
| 35 | 204 | Nicole MERTZ | Velo Classic pb Stans NoTubes | 1:02:46. | s.t. |
| 36 | 171 | Danielle CLARK | RTO National Womens Cycling | 1:02:47. | s.t. |
| 37 | 182 | Breanne NALDER | UCI WPT: Visit Dallas DNA Pro C | 1:02:47. | s.t. |
| 38 | 159 | Caroline BAUR | RMV Elgg | 1:02:47. | s.t. |
| 39 | 225 | Briana CLARK | First Internet Bank Cycling Tea | 1:02:47. | s.t. |
| 40 | 188 | Kimberly PETTIT | Papa Johns Racing pb TREK | 1:02:48. | s.t. |
| 41 | 181 | Melinda MCCUTCHEON | UCI WPT: Visit Dallas DNA Pro C | 1:02:48. | s.t. |
| 42 | 183 | Kathryn DONOVAN | UCI WPT: Visit Dallas DNA Pro C | 1:02:48. | s.t. |
| 43 | 199 | Madeleine PARK | UCI WPT: Team TIBCO-Silicon Val | 1:02:49. | s.t. |
| 44 | 205 | Ashley BARSON | Rise Racing | 1:02:49. | s.t. |
| 45 | 211 | Katherine SHIELDS | OrthoCarolina Winston Womens C | 1:02:50. | 19 |
| 46 | 156 | Mary Emily DAVIDSON | Team Pursuit | 1:04:48. | 2:17 |
| 47 | 219 | Colleen GULICK | PICKLE JUICE PRO CYCLING TEAM | 1:04:48. | s.t. |
| 48 | 218 | Nicolle BRUDERER | UCI WPT: Team TIBCO-Silicon Val | 1:04:48. | s.t. |
| 49 | 224 | Jane TULLIS | Frazier Cycling Juniors | 1:04:48. | s.t. |
| 50 | 153 | Diana RAMOS | OrthoCarolina Winston Womens C | 1:04:49. | s.t. |
| 51 | 166 | Jennifer HALE | BirchwoodGIS | 1:04:49. | s.t. |
| 52 | 169 | Sarah GUILBERT | RTO National Womens Cycling | 1:04:49. | s.t. |
| 53 | 220 | Debbie PROUTY | Hilton Head CyclingRobson Foren | 1:04:49. | s.t. |
| 54 | 213 | Victoria KANIZER | OrthoCarolina Winston Womens C | 1:04:49. | s.t. |
| 55 | 157 | Barbora STELLA | Sorella Cycling pb Hincapie Spo | 1:04:49. | s.t. |
| 56 | 170 | Jennifer WAKEMAN | RTO National Womens Cycling | 1:04:49. | s.t. |
| 57 | 172 | Susan JONES - MORGAN | RTO National Womens Cycling | 1:04:49. | s.t. |
| 58 | 151 | Stephanie SMITH | | 1:04:49. | s.t. |
| 59 | 162 | Maria DANKER | Hicks Orthodontics Womens Raci | 1:04:49. | s.t. |
| 60 | 198 | Jennifer TETRICK | UCI WPT: Team TIBCO-Silicon Val | 1:04:49. | s.t. |
| 61 | 163 | Teresa O SULLIVAN | Dornier Racing | 1:04:49. | s.t. |
| 62 | 189 | Payten MANESS | Papa Johns Racing pb TREK | 1:04:49. | s.t. |
| 63 | 184 | Katie CLOUSE | UCI WPT: Visit Dallas DNA Pro C | 1:04:49. | s.t. |
| 64 | 154 | Helen MITCHELL | OrthoCarolina Winston Womens C | 1:04:49. | s.t. |
| 65 | 161 | Lindsey DURST | Hollander Cycling pb ISCorp | 1:04:49. | s.t. |
| DNF | 217 | Jennifer SCHUBLE | Infinity Cycling Team | | |
| DNF | 175 | Liza RACHETTO | UCI WPT: Hagens Berman | Superm | | |
| DNF | 203 | Kristen ARNOLD | Velo Classic pb Stans NoTubes | | |
| DNF | 160 | Loren MORGAN | Prima Tappa Cycling pb Mansfiel | | |
| DNF | 193 | Katherine SHERWIN | UCI WPT: UnitedHealthcare Pro C | | |
| DNF | 195 | Lauretta HANSON | UCI WPT: UnitedHealthcare Pro C | | |
| DNF | 200 | Krista DOEBEL-HICKOK | UCI WPT: Cylance Pro Cycling | | |
| DNF | 223 | Courteney LOWE | Mellow Mushroom pb Psimet | | |
| DNS | 998 | Kassandra KRIARAKIS | Rise Racing | | |
| DNS | 999 | Cinthia LEHNER | RTO National Womens Cycling | | |
