| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 143 | TEDDERGREEN Starla | Hagens Berman | Supermint | 1:00:07. | 1:00:07 |
| 2 | 111 | BRECK Holly | Sho-Air TWENTY20 | 1:00:07. | s.t. |
| 3 | 104 | AUDRAIN Ivy | Hagens Berman | Supermint | 1:00:08. | s.t. |
| 4 | 122 | HEINTZ Mandy | Visit Dallas DNA Pro Cycling | 1:00:08. | s.t. |
| 5 | 135 | ROSE Claire | Visit Dallas DNA Pro Cycling | 1:00:08. | s.t. |
| 6 | 150 | ZAVETA Erica | Cylance Pro Cycling | 1:00:08. | s.t. |
| 7 | 119 | GARDNER Illi * | Folsom Bike/Trek | 1:00:08. | s.t. |
| 8 | 117 | DOEBEL-HICKOK Krista | Cylance Pro Cycling | 1:00:08. | s.t. |
| 9 | 141 | SOTO Yusse | Team Illuminate | 1:00:08. | s.t. |
| 10 | 139 | SCHNEIDER Skylar * | Team Illuminate | 1:00:08. | s.t. |
| 11 | 103 | ARREOLA NAVARRO Sofia | Sho-Air TWENTY20 | 1:00:08. | s.t. |
| 12 | 107 | BAYER Lindsay | Hagens Berman | Supermint | 1:00:09. | s.t. |
| 13 | 138 | SCHNEIDER Samantha | Team Illuminate | 1:00:09. | s.t. |
| 14 | 116 | CORDOVA Lisa | Cycle Sport-Specialized pb Musc | 1:00:09. | s.t. |
| 15 | 133 | POWLESS Shayna | Sho-Air TWENTY20 | 1:00:09. | s.t. |
| 16 | 128 | MUEGGE Hanna | Visit Dallas DNA Pro Cycling | 1:00:09. | s.t. |
| 17 | 146 | THOMAS Leah | Sho-Air TWENTY20 | 1:00:09. | s.t. |
| 18 | 137 | SAYLE Clare | Jakroo presented by Chrome Indu | 1:00:09. | s.t. |
| 19 | 101 | ALDERETE Megan | Hagens Berman | Supermint | 1:00:10. | s.t. |
| 20 | 129 | MYERS Lindsay | | 1:00:10. | s.t. |
| 21 | 148 | WONG Melanie | Folsom Bike/Trek | 1:00:10. | s.t. |
| 22 | 112 | CAMERON Amy | Folsom Bike/Trek | 1:00:10. | s.t. |
| 23 | 102 | ARNOLD Kristen | Velo Classic pb Stans NoTubes | 1:00:10. | s.t. |
| 24 | 145 | TETRICK Jennifer | Team TIBCO-Silicon Valley Bank | 1:00:10. | s.t. |
| 25 | 123 | LANGDON Rachel | Team Illuminate | 1:00:10. | s.t. |
| 26 | 132 | OTTO HAMEL Liz | Folsom Bike/Trek | 1:00:11. | s.t. |
| 27 | 134 | RACHETTO Liza | Hagens Berman | Supermint | 1:00:11. | s.t. |
| 28 | 105 | AXELL Marissa | JL Velo Cycling Team pb The Fre | 1:00:11. | s.t. |
| 29 | 149 | WRYE-SIMPSON Brenna | LA Sweat | 1:00:12. | s.t. |
| 30 | 124 | LUEBKE Jennifer | Visit Dallas DNA Pro Cycling | 1:00:12. | s.t. |
| 31 | 114 | CERRA Jessica | Hagens Berman | Supermint | 1:00:12. | s.t. |
| 32 | 113 | CELSO Jo | Jakroo presented by Chrome Indu | 1:00:12. | s.t. |
| 33 | 144 | TETRICK Alison | Cylance Pro Cycling | 1:00:12. | s.t. |
| 34 | 136 | RUBLE Megan | Cycle Sport-Specialized pb Musc | 1:00:12. | s.t. |
| 35 | 127 | MOAK Summer * | Sho-Air TWENTY20 | 1:00:13. | s.t. |
| 36 | 108 | BOBECK Jessica | Team Illuminate | 1:00:13. | s.t. |
| 37 | 131 | ORTON Beth Ann | Hagens Berman | Supermint | 1:00:14. | s.t. |
| 38 | 120 | GIESCHEN Julia | STINNER BLACK SHEEP RACING | 1:00:14. | s.t. |
| 39 | 118 | DONOVAN Kathryn | Visit Dallas DNA Pro Cycling | 1:00:14. | s.t. |
| 40 | 130 | NALDER Breanne | Visit Dallas DNA Pro Cycling | 1:00:15. | s.t. |
| 41 | 115 | CHALIER Annick | Fast Fun Nice RacingTeam pb Wat | 1:00:16. | 9 |
| 42 | 140 | SEIGLE Amanda | Jakroo presented by Chrome Indu | 1:00:18. | 11 |
| 43 | 121 | GREGG Amity | Jakroo presented by Chrome Indu | 1:03:19. | 3:12 |
| 44 | 125 | MCGOWAN Ayesha | Jakroo presented by Chrome Indu | 1:04:07. | 4:00 |
| 45 | 110 | BONNINGTON Christina | JLVelo Cycling Team pb The Free | 1:04:23. | 4:16 |
| 46 | 126 | MILLARD Lexie | Team Illuminate | 1:04:57. | 4:50 |
| 47 | 109 | BOLLELLA Allie | JLVelo Cycling Team pb The Free | 1:05:00. | 4:53 |
| 48 | 106 | BAUMGARDNER Jessica | STINNER BLACK SHEEP RACING | 1:07:23. | 7:16 |
| DNS | 142 | STUMHOFER Gretchen | Sho-Air TWENTY20 | | |
| DNS | 147 | VAN HOUWELING Molly | JLVelo Cycling Team pb The Free | | |
