| Rank | Bib | Name | Affiliation | Time | Gap | Category |
| 1 | 244 | Marlies Mejias Garcia | UCI CTW: Virginias Blue Ridge TWENTY24 | 1:07:36.5 | 1:07:36.5 | Women Pro/1/2 |
| 2 | 222 | Alexis Ryan | L39ION OF LOS ANGELES | 1:07:37.3 | s.t. | Women Pro/1/2 |
| 3 | 223 | Kendall Ryan | L39ION OF LOS ANGELES | 1:07:37.6 | s.t. | Women Pro/1/2 |
| 4 | 218 | Kimberly Lucie | DNA Pro Cycling | 1:07:37.9 | s.t. | Women Pro/1/2 |
| 5 | 229 | Mariana Valadez | LA Sweat | 1:07:38.0 | s.t. | Women Pro/1/2 |
| 6 | 210 | Kaitlyn Agnew | ButcherBox Cycling p/b LOOK | 1:07:38.1 | s.t. | Women Pro/1/2 |
| 7 | 224 | Skylar Schneider | L39ION OF LOS ANGELES | 1:07:38.1 | s.t. | Women Pro/1/2 |
| 8 | 234 | Taylor Kuyk-White | Miami Nights | 1:07:38.8 | s.t. | Women Pro/1/2 |
| 9 | 236 | Brittany Parffrey | Miami Nights | 1:07:39.2 | s.t. | Women Pro/1/2 |
| 10 | 211 | Gillian Bennett | ButcherBox Cycling p/b LOOK | 1:07:39.4 | s.t. | Women Pro/1/2 |
| 11 | 217 | Gabrielle Lehnert | DNA Pro Cycling | 1:07:39.8 | s.t. | Women Pro/1/2 |
| 12 | 209 | Alexi Ramirez | Blazers | 1:07:40.4 | s.t. | Women Pro/1/2 |
| 13 | 204 | Aubrey Drummond | Austin Aviators | 1:07:41.2 | s.t. | Women Pro/1/2 |
| 14 | 240 | Olivia Reeve | Road Crew Coffee Racing | 1:07:41.7 | s.t. | Women Pro/1/2 |
| 15 | 200 | Regina Doty | | 1:07:42.0 | s.t. | Women Pro/1/2 |
| 16 | 226 | Emily Flynn | LA Sweat | 1:07:44.1 | 7.6 | Women Pro/1/2 |
| 17 | 202 | Hanna Coppens | Apex Cycling Team | 1:07:48.8 | 12.3 | Women Pro/1/2 |
| 18 | 205 | Mary joyce Monton | Austin Aviators | 1:07:52.5 | 16.0 | Women Pro/1/2 |
| 19 | 219 | Harriet OWEN | DNA Pro Cycling | 1:07:52.8 | s.t. | Women Pro/1/2 |
| 20 | 242 | Sofia Arreola | UCI CTW: Virginias Blue Ridge TWENTY24 | 1:07:54.9 | 18.4 | Women Pro/1/2 |
| 21 | 212 | Paige Kostanecki | ButcherBox Cycling p/b LOOK | 1:07:56.4 | 19.9 | Women Pro/1/2 |
| 22 | 213 | Rachel Plessing | ButcherBox Cycling p/b LOOK | 1:08:04.8 | 28.3 | Women Pro/1/2 |
| 23 | 245 | Danielle Morshead | UCI CTW: Virginias Blue Ridge TWENTY24 | 1:08:57.0 | 1:20.5 | Women Pro/1/2 |
| 24 | 221 | Julyn Aguila | L39ION OF LOS ANGELES | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 25 | 225 | Gabriella Arnold | LA Sweat | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 26 | 230 | Ashley Weaver | LA Sweat | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 27 | 237 | Ashley King | Orion Racing p/b Borah Teamwear | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 28 | 216 | Elizabeth Harden | Denver Disruptors | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 29 | 239 | Katherine Lin | Road Crew Coffee Racing | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 30 | 227 | Monica Merced | LA Sweat | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 31 | 206 | Esma Taylor | AUTOMATIC | ABUS Racing | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 32 | 246 | Allison Mccurry | Instafund Pro Cycling | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 33 | 203 | Sarah Weihe | Apex Cycling Team | 1:08:58.0 | s.t. | Women Pro/1/2 |
| 34 | 201 | Jess Hamilton | Apex Cycling Team | 1:08:58.1 | s.t. | Women Pro/1/2 |
