| Rank | Bib | Name | Affiliation | Time | Split Time | Laps |
| 1 | 512 | Jen TILLMAN | Knobby By Nature | 41:12. | 10:21. | 4 |
| 2 | 514 | Denelle GRANT | Flying Frog Racing/coqui Cycler | 41:59. | 10:45. | 4 |
| 3 | 511 | Mackenzie YATES | Ncvc/unitedhealthcare | 42:53. | 10:53. | 4 |
| 4 | 854 | Ava WHITE | | 43:23. | 10:25. | 4 |
| 5 | 855 | Juhi SHAH | Meta.cc | 43:37. | 10:26. | 4 |
| 6 | 516 | Katie SMITH | Cardinal Bicycle | 44:00. | 11:22. | 4 |
| 7 | 858 | Bridget CIAMBOTTI | Miller School Of Albemarle | 44:02. | 10:41. | 4 |
| 8 | 866 | Nora DAUSCH | Rock Creek Velo | 44:03. | 10:22. | 4 |
| 9 | 587 | Elizabeth HARLOW | | 45:38. | 11:52. | 4 |
| 10 | 852 | Kristin REECE | Nomad Cycling Services | 45:53. | 11:09. | 4 |
| 11 | 851 | Tonya WHITE | Aw5 Racing | 46:11. | 11:23. | 4 |
| 12 | 515 | Sarah LUKENS | American Flyers Racing | 46:21. | 12:13. | 4 |
| 13 | 863 | Kristi WALKER | Sweet Spot Cycling | 46:28. | 11:36. | 4 |
| 14 | 861 | Ali OTTO | Nctc | 46:42. | 11:26. | 4 |
| 15 | 586 | Irina SARKISOV | Crosshairs Cycling | 47:00. | 11:48. | 4 |
| 16 | 513 | Dori BUCKETHAL | Catoctin Cycling Club | 47:04. | 11:57. | 4 |
| 17 | 588 | Melissa PAQUETTE | Stk Cycling | 47:34. | 11:46. | 4 |
| 18 | 856 | Sharon SQUASSONI | | 47:41. | 11:40. | 4 |
| 19 | 591 | Jennifer RHODES | Stk Cycling | 36:40. | 12:32. | 3 |
| 20 | 857 | Rebecca IRWIN KENNEDY | Rock Creek Velo | 37:18. | 12:12. | 3 |
| 21 | 876 | Carolyn WRIGHT | | 37:28. | 11:58. | 3 |
| 22 | 859 | Samantha GOLDENSTEIN | Us Military Endurance Sports | 37:29. | 12:22. | 3 |
| 23 | 864 | Brooke EVANS | Nctc | 37:36. | 12:21. | 3 |
| 24 | 593 | Tanya HARRIS | Team Spoke Cycles | 37:40. | 12:30. | 3 |
| 25 | 589 | Julie PAISANT | Mock Orange Racing | 37:51. | 12:39. | 3 |
| 26 | 592 | Nicole BRANDT | | 38:27. | 13:19. | 3 |
| 27 | 880 | Moira BARRETT | Psimet Racing | 38:36. | 12:10. | 3 |
| 28 | 882 | Susan KEFER | | 39:26. | 12:48. | 3 |
| 29 | 862 | Barbara HANEY | Ncvc/initedhealthcare | 39:38. | 12:55. | 3 |
| 30 | 594 | Teresa BRUSADIN | Thr Cycling | 40:46. | 13:36. | 3 |
| 31 | 879 | Christi BRITT | Betty Squad Racing | 41:15. | 13:37. | 3 |
| 32 | 873 | Shelley MOLNAR | Hammercross | 29:04. | 14:00. | 2 |
| 33 | 869 | Megan BAXTER | Constellation Cycling | 29:12. | 14:23. | 2 |
| 34 | 874 | Jo MARTIN | Team Sb | 29:44. | 14:37. | 2 |
